ArticleViewAbstractPharmacognosy Journal,2019,xx-xx.DOI:10.5530/pj.2017.4.xType:Original ArticleVirtual Screening of Indonesian Herbal Database for Discovery of Procaspase-3 Activators Using Autodock and Autodock VinaRezi Riadhi Syahdi, Ayu Annissa, and Arry Yanuar Rezi Riadhi Syahdi, Ayu Annissa, Arry Yanuar* Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, INDONESIA. Abstract:Objective: Cancer is a disease where body cell grows abnormal, spread to every part of human body. Previous studies have found excessive expression of Procaspase-3 on cancer that must be activated to Caspase-3 to induce apoptotic in cells. Methods: Virtual screening of Indonesian Herbal Database was carried out to discover Procaspase-3 activators. This study was validated using enrichment factor (EF), receiver operating characteristics (ROC) area under curve (AUC) parameters. Among 1412 compounds were screened using Autodock and Autodock Vina software. Results: The virtual screening results using Autodock obtained the best ten compounds with binding energy -8.28 ~ -9.31 kcal/mol and Autodock Vina obtained the best ten compounds with binding energy -8.1 ~ -8.8 kcal/mol. Both virtual screening software showed two compounds in common, i.e., betulinic acid and maslinic acid. Conclusion: Betulinic acid interacts with Leu136A, Lys137A, Tyr195A and Pro201 residues in Autodock and Autodock Vina. While maslinic acid interacts with Leu136A, Lys137A and Pro201 residues in Autodock and Autodock Vina Keywords:Apoptotic, Cancer, Herbal, Procaspase-3 activator, Virtual Screening Images Three-dimensional structure of Procaspase-3 macromolecule, PDB ID: 4JR0.