ACE Inhibitory Activity, Total Phenolic and Flavonoid Content of Pereskia saccharose Griseb. Leaves Extract

Introduction: Angiotensin-converting enzyme inhibitors (ACEi) are drugs that can control hypertension. Pereskia saccharose Griseb. leaves have been used traditionally as antihypertensive. Objective: The objective of this study was to determine the antihypertensive activity through inhibition of ACE activity, the total phenolic content and total flavonoid content of the ethanolic extract of Pereskia saccharose Griseb. leaves and its fractions. Methods: Extraction was done by maceration with 80% ethanol and fractionation performed by liquid-liquid partition. Results: In vitro ACE inhibitory activity assay of the ethanolic extract using ACE Kit-WST Dojindo had IC₅₀ value of 3.448 μg/mL and ethyl acetate fraction had IC₅₀ value of 1.714 x 10-3 μg/mL. Ethyl acetate contained the highest amounts of both TPC (72.991 ± 0.932 mg GAE/g sample) and TFC (61.337 ± 1.612 mg QE/g sample). Conclusion: The results suggest that Pereskia saccharose Griseb. possess ACE inhibitory activity.