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Acute Toxicity Tests of Fermented Robusta Green Coffee Using Zebrafish Embryos (Danio rerio)

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Examples of zebrafish conditions. Healthy: (a) negative control 24 hpf, (b) negative control 96 hpf, (c) Positive control (Dichloroaniline 4 mg/l), Characters appeared in the zebrafish embryo after 96 hpf (observation zebrafish embryo classification deemed dead according to the OECD, 2013), (d) 500 ppm 24 hpf (lack of somit formation), (e) 1000 ppm 96 hpf (pericardial edema), (f) 300 ppm 96 ppm (scoliosis)

Cite This Article

Vancouver Style ::
Narko T, Wibowo MS, Damayanti S, Wibowo I. Acute Toxicity Tests of Fermented Robusta Green Coffee Using Zebrafish Embryos (Danio rerio). Pharmacognosy Journal. 2020;12(3):485-492 .PDF icon PDF (2.21 MB)