ELISA Test on Cordia myxa L. Leaf Extract for alpha-Glucosidase Inhibitor

Aimed: Determine the potential of Cordia myxa L. leaf on inhibited α-glucosidase. Material: ELISA Kit, Ethanol 96%, Colomn Chromatography, n-hexane, ethyl acetate, Glocobay®.  Method: Sample from Cordia myxa L. leaf extracted by ethanol 96% then evaporated to get the sticky extract. The sticky extract of Cordia myxa L. leaf fractionated by column chromatography with n-hexane, n-hexane: ethyl acetate (90:10; 80:20; 75:25; 70:30; 65:35; 60:40; 55:45; 50:50) Assay: The fractions assayed by ELISA (Enzyme-Linked Immunosorbent Assay) with acarbose (Glucobay ®) as the comparator. Result: The results showed that the n-hexane fraction is the highest potency on inhibited α-glucosidase with the noncompetitive mechanism. The IC₅₀ of n-hexane fraction is 0.53 ppm been while the acarbose is 6.85 ppm. Conclusion: The n-hexane fraction of Cordia myxa L. leaf has the highest potency to use for possible decrease blood glucose level.