Objective: The aim of the present study was to investigate the hepatoprotective effect of Livplus (a polyherbal formulation) against CCl4-induced hepatotoxicity in rats. Methods: Hepatotoxicity was induced in rats by i.p. injection of CCl4 once three days for 14 days. Livplus or Silymarin was administered along with CCl4 and the biochemical parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkalinephosphatase (ALP), total bilirubin (TB), direct bilirubin, total protein (TP), gamma-glutamyl transferase (GGT), total cholesterol (TC) and triglycerides (TG) were estimated. Furthermore, biomarkers of oxidative stress such as MDA levels, Glutathione contents, SOD and catalase activity in liver tissue were estimated. Results: Treatment with Livplus significantly reduced the elevated levels of ALT, AST, ALP, bilirubin (direct and total), GGT, TC, TG and increased levels of TP compared to CCl4 control rats. The treatment with Livplus also showed a significant increase in glutathione contents, SOD and catalase activity and a decrease in MDA levels compared to CCl4 control rats. Conclusion: The finding of present study indicates that Livplus showed a potential hepatoprotective activity. These results support the traditional use of Livplus in the treatment of liver disorders.