Effects of Hydrolyzed Marine Collagen Peptide Supplementation Combined with Phytonutrients on Skin Biophysical Parameters and Extracellular Matrix Biomarkers in Thai Women: A Prospective Clinical Study

Background: Skin aging involves progressive deterioration of extracellular matrix components. This study investigated the effects of hydrolyzed marine collagen peptide supplementation combined with phytonutrients on skin biophysical parameters and dermal biomarkers in Thai women. Methods: Sixtythree Thai women aged 40–60 years received daily supplementation (10,000 mg marine collagen peptides, Chlorella, grape seed extract, rice ceramides) for 12 weeks. Assessments included Antera 3D imaging, Cutometer elasticity measurements, Tewameter barrier function evaluation, and Corneometer hydration analysis. A subset (n=20) underwent suction blister biopsy for dermal biomarker analysis. Results: Significant improvements were observed in Cutometer parameters: R5 net elasticity (p<0.001), R6 viscoelasticity (p=0.018), and R7 biological elasticity (p=0.003). Transepidermal water loss (TEWL) decreased by 12.1% (p=0.007). Antera 3D analysis revealed a significant increase in wrinkle width (p=0.021), indicating wrinkle flattening. Biomarker analysis showed significant increases in fibrillin-1 (+8.34%, p=0.005) and biglycan (+22.91%, p=0.036). Glogau photoaging classification improved in 23.8% of participants. Conclusions: Marine collagen peptide supplementation combined with phytonutrients significantly improved skin elasticity and barrier function, with molecular evidence of extracellular matrix enhancement in Thai women.