Subacute Toxicity Test of Hydrocotyle Sibthorpioides Lam. Extract on Histopathological Images of Liver and Kidney of White Male Mice

Introduction: Hydrocotyle sibthorpioides Lam. in Indonesia known as pegagan embun. It has been used to increase the immune system and has been shown to have immunostimulating, anti-inflammatory and hematopoietic effects. However, there is no scientific evidence that shows this plant is safe for long-term use. Based on that circumstance, this study aimed to measure the safety of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) ethanol extract activities on liver and kidney histopathology. Aim: The study aimed to measure the safety of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) ethanol extract activities on liver and kidney histopathology. Material and Method: Ethanol extract used because all the active compounds in plants extracted as a whole, and it cheaper and more efficient in the extraction process. Determine as many thirty-six white male mice as test animals and separate them into eight treatment groups. The administrated ethanol extract of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) at doses of 7, 35, and 150 mg/kg BW for 7, 14, and 21 days. On days 8^th, 15^th, and 22^nd, three white male mice collected from each treatment group and collected their liver and kidney. The data analysed used a T-test with IBM SPSS type 24. Result: LD50 of ethanol extract of Hydrocotyle sibthorpioides Lam. > 15,000 mg/kg means practically not toxic. The results showed that the administration of extract Hydrocotyle sibthorpioides Lam. for 7, 14, and 21 days showed a non-significant effect on any histological damage to the liver of male white mice at doses of 7 and 35 mg/kg BW (normal histology). The non-significant effect also occurs at150 mg/kg BW for 7 days; however, it caused mild damage at a dose of 150 mg/kg BW for 14 days and moderate damage at 150 mg/kg BW for 21 days. In renal histopathology, doses of 7 mg/kg BW. for 7, 14, and 21 days showed normal histology and doses of 35 mg/kg BW for 7, 14, and 21 days showed minimal damage. The administration at doses of 150 mg/kg BW for 7 days showed mild damage, while a dose of 150 mg/kg BW for 14 and 21 days showed moderate damage. Conclusion: It concluded that the administration of extract of Hydrocotyle sibthorpioides Lam. did not cause severe damage to the histology of the liver and kidneys of white male mice.