ArticleViewAbstractPharmacognosy Journal,2015,7,3,157-164.Published:13th Feb, 2015Type:Original ArticleEffectiveness of Sidr Honey on the prevention of ethanol-induced gatroulcerogenesis: role of antioxidant and antiapoptotic mechanismManal Mohamed Elhassan Taha, Siddig Ibrahim Abdelwahab, Rashad Elsanousi, Bassem Y. Sheikh, Mahmood Ameen Abdulla, Saif Eldeen Babiker, Husham Elraih, and Eldaw Mohamed Manal Mohamed Elhassan Taha1*, Siddig Ibrahim Abdelwahab1, Rashad Elsanousi1, Bassem Y. Sheikh2, Mahmood Ameen Abdulla3, Saif Eldeen Babiker1, Husham Elraih1, Eldaw Mohamed1 1Medical Research Centre, Jazan University, Jazan, Saudi Arabia2MABL Scientific Chair, Department of Surgery, College of Medicine, Taibah University, Saudi Arabia3Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Malaysia.Abstract:Background: Sider (Ziziphus spina-christi (L.) Desf.) Honey has been used for the treatment of gastrointestinal disorders including peptic ulcer. Aim of the study: The mechanism of the antiulcer effect of sider honey was studied placing emphasis on its role to block oxidative damage and apoptosis during ethanol-induced gastric ulceration in rats. The mechanism of the antiulcer effect of sider honey was studied placing emphasis on its role to block oxidative damage and apoptosis during ethanol-induced gastric ulceration in rats. Materials and methods: Experimental animals were orally treated with sidr honey (100, 250 and 500 mg/kg, respectively) or omeprazole and subsequently exposed to 95%ethanol (5 mL/Kg, orally) to induce acute gastroulcerogenesis. Effectiveness of sidr honey was evaluated using ulcer index, pH of gastric juice, mucus content, morphological analyses, glutathione assay and malondialdehyde level. The anti-apoptotic role of sidr honey was studied using immunohistochemical staining of gastric tissues using monoclonal antibodies of Bax pathway. Results: Dose-response studies in ethanol-induced ulcer indicate that sidr honey significantly blocks gastric lesions at lower dose (100 mg/kg). Lipid peroxidation and glutathione depletion were significantly inhibited by sidr honey. Sidr honey modulated the immuno-expression of mitochondrial associated protein (Bax). Conclusion: Thus, sider honey plays a considerable role in gastro protection by acting as a potent antioxidant and antiapoptotic agent. Future study is required to explore its potential clinical usage.Key words: Antiapoptotic Mechanism, Antioxidant Agents, Gastro protection, Monofloral Nectar, Sidr Honey.Keywords:Antiapoptotic Mechanism, antioxidant agents, Gastro protection, monofloral nectar, Sidr Honey.