ArticleViewAbstractPharmacognosy Journal,2019,11,4,674-677.DOI:10.5530/pj.2019.11.106Published:July 2019Type:Original ArticleIn vitro Antidiabetic Activity of Methanolic Leaf Extract of Indianthus virgatus (Roxb.) Suksathan and Borchs by Glucose Uptake MethodSangeetha DN, and S. Rajamani Sangeetha DN1,*, S. Rajamani2 1Research Scholar, Bharathiar University, Coimbatore, Tamil Nadu, INDIA. 2Associate Professor, Department of Botany, St. Josephs’ Post Graduate and Research Centre, Bengaluru, Karnataka, INDIA. Abstract:Medicinal plants play a key role to cure many diseases from time immemorial. The usage of medicinal plants in traditional medicinal system is the vital process of India. Diabetes Mellitus is a systemic metabolic disease characterized by hyperglycemia, abnormal elevated levels of lipid and fat in blood and hypoinsulinaemia. The current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. Thus, introduction of alternative and complementary medicine is now in picture. Objective: The main objective of this work is to evaluate the in vitro anti diabetic activity of methanolic extracts of Indianthus virgatus (Roxb.) Suksathan and Borchs in skeletal muscle cell line. Methods: The in vitro cytotoxicity was performed for leaf extract (Methanol) on L-6 (Rat skeletal muscle) cell line to find toxic concentration of the leaf extract by MTT assay. Glucose uptake activity of test substance was determined in differentiated L-6 cells. Results: In Glucose uptake assay, Methanol Extract exhibited moderate toxicity to skeletal muscle cell line and glucose uptake assay it shows dose dependent glucose uptake. Glucose uptake rate increased with the increasing concentration of the leaf extract. Conclusion: The results of the current study clearly demonstrated the antidiabetic potency of methanolic leaf extract obtained from Indianthus virgatus (Roxb.) Suksathan and Borchs. under in vitro model. Keywords:Antidiabetic activity, In vitro cytotoxicity, Indianthus virgatus (Roxb.) Suksathan and Borchs, L-6 cell line, Methanolic leaf extract, MTT Assay, Skeletal muscle Cell Lines, Therapeutic agentsView:PDF (600.3 KB) PDF Images Cytotoxicity of test substance on L-6 cell line ‹ Anti-inflammatory Effect of the Aqueous Fruit Pulp Extract of Tamarindus indica Linn in Lipopolysaccharide-Stimulated Macrophages up The Effect of Noni Fruit Extract (Morinda citrifolia L.) in Gingivitis Patient ›