Objectives: To Investigate the phytochemicals present in Bryonopsis laciniosa fruit. Methodology: Qualitative, Quantitative screening, Compound Identification by UV-Visible method and identification of functional group of the active chemical components were followed by standard procedures. Result: The results showed the presence of phytonutrients like reducing sugar, terpenoids, triterpenoids, aminoacids, anthroquinone, polyphenols, glycosides, anthocyanins, tannins, coumarins, emodins, saponins, total alkaloids, total flavonoids, lignin and serpentine. These substances may be responsible for the health related properties of the plant which are based on antioxidant, anticancer, antipyretic, antiaphoretic, antidiabetic, anti-inflammation, antiheamatisum, antimicrobial and antiviral activity. Conclusion: This study supports the popular use of Bryonopsis laciniosa fruit in preparation of various pharmaceutical formulations for human welfare.