ArticleViewAbstractPharmacognosy Journal,2022,14,2,388-396.DOI:10.5530/pj.2022.14.50Published:April 2022Type:Research Article Mechanism of the Bioactive Sargassum cristaefolium in Inhibiting Inflammatory Mediators in a Nitroglycerin-Induced Migraine Model in RatsOlivia Mahardani Adam, Jusak Nugraha, Muhammad Hamdan, and Agus Turchan Olivia Mahardani Adam1,2, Jusak Nugraha3,*, Muhammad Hamdan4, Agus Turchan5 1Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA. 2Department of Neurology, Faculty of Medicine, Universitas Hang Tuah, Surabaya, INDONESIA. 3Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA. 4Department of Neurology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA. 5Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA. Abstract:Background: Migraine headaches are a form of sterile neurogenic inflammation. The sterile inflammatory process of the trigeminal nerve releases the vasoactive neuropeptide CGRP which stimulates the release of inflammatory mediators. In the incidence of migraine there is an increase in TNF-α and IL-10. Sargassum cristaefolium ethanol extract contains flavonoids, alkaloids, triterpenoids, steroids, and tannins, which has analgesic and anti-inflammatory function. Method: Sargassum cristaefolium was extracted using maceration method with 70% ethanol as solvent. Animal models were divided into 5 groups and given NTG induction 5 times with 1 day intervals, treated for 3 weeks. All data were analyzed using IBM SPSS version 26.0. Results: Sargassum cristaefolium ethanol extract - CGRP levels β: -0.26, p: 0.17; Sargassum cristaefolium ethanol extract - CGRP expression β: -0.04, p: 0.85; Sargassum cristaefolium ethanol extract - TNF-α levels β: -0.63, p: 0.01; Sargassum cristaefolium ethanol extract - TNF-α expression β: -0.40, p: 0.04; Sargassum cristaefolium ethanol extract - IL-10 levels β: 0.77, p: 0.00; Sargassum cristaefolium ethanol extract - IL-10 expression β: 0.45, p: 0.01. Conclusions: A significant path between the administration of Sargassum cristaefolium ethanol extract and a decrease in TNF-α and an increase in IL-10. But the effect of giving Sargassum cristaefolium ethanol extract on CGRP levels did not have a significant relationship. Keywords:Inflammatory mediator, Migraine, Nitroglycerin, Sargassum cristaefoliumView:PDF (748.14 KB) PDF Images Mechanism of the Bioactive Sargassum cristaefolium in Inhibiting Inflammatory Mediators in a Nitroglycerin-Induced Migraine Model in Rats ‹ The Potential of Moringa (Moringa oleifera Lamk) Seed Oil as Anti- Alopecia up Optimization of the Ultrasound Assisted Extraction of Phaleria macrocarpa (Scheff.) Boerl. Fruit Peel and its Antioxidant and Anti-Gout Potential ›