Context: High cholesterol in the blood is a risk factor for atherosclerosis that causes various diseases. The main pharmacologic intervention to reduce cholesterol levels is inhibiting the HMG-CoA reductase enzyme. One of the genera of Garcinia, Garcinia dulcis, has potential as an anti-cholesterol. Based on chemotaxonomy, Garcinia latissima Miq. is also estimated to have a potency as anti-cholesterol. Aims: This study aims to test the inhibition of HMG-CoA reductase water extract activity of G. latissima fruit flesh with different duration of infusions. Materials and Methods: Garcinia latissima Miq. mesocarp was extracted using infusion method with different duration of infusions. Each of extracts was tested the inhibitory activity of HMG-CoA reductase as well as the determination of total flavonoid and total phenol content. In addition, the simplicia of the mesocarp of G.latissima Miq. will be made as a herbal tea and a hedonic test is performed to find out the degree of liking for the tea. Result: The test results showed the inhibitory activity of 100 ppm G. latissima Miq. mesocarp water extract with infusion for 5, 10 and 15 min respectively 11.32; 29.02; 13.03%. The 10 min extract with the largest enzyme inhibition had total flavonoids content of 31.24 mg QE / gram extract and total phenol content of 4.64 mg GAE/ gram extract. The result of the hedonic test for the colour, aroma, flavour of herbal tea formula A respectively 30; 30; 20% and formula B respectively for 40; 33.3; 50%. Conclusion: The water extract of G.latissima Miq mesocarp has a low potency in HMG-CoA reductase inhibitory activities.