Introduction: Safoof-e-Makhana (SM) is a Unani polyherbal powder formulation used to treat Sexual disorders. Formulation consists of following herbs Orchis latifolia, Hygrophila auriculata, Asparagus racemosus and Elettaria cardamomum. The main aim of this study was to standardize Safoof-e-Makhana (SM), on the basis of organoleptic characters and physico-phytochemical analysis. Methods: The drugs were cleaned, dried in shade and powdered by passing through sieve # no. 80 as per the method described in UPI / National Formulary of Unani Medicine. This Safoof formulation was evaluated using physicochemical tests: powder characterization, extractive value, alcohol and water soluble matter, Ash value, LOD at 1050C, pH and HPTLC fingerprinting. Statistical analysis used: Mean ± SEM. Results: Organoleptic characters of the formulation are light brown colour, characteristic odour, sweet taste and moderately fine texture. Physicochemical parameters displayed water soluble extractive (21.83 ± 0.08), alcohol soluble extractive (7.87 ± 0.09), total ash (4.76 ± 0.08), acid insoluble ash (3.36 ± 0.01), water soluble ash (0.89 ± 0.25), LOD at 105oC (11.38 ± 0.34), pH of 1% and 10% solution were 6.6 ± 0.1 and 6.0 ± 0.1 respectively. Phytochemical qualitative analysis displayed presence of alkaloids, tannins, flavanoids, steroids, terpenoids, carbohydrates, volatile oil. HPTLC fingerprinting data was also set in. Conclusion: The standardization of this formulation was done and the data obtained would be used as a standard for future reference.