Introduction: Sumbul-al–Teeb (Nardostachys jatamansi) is a well known traditional medicinal plant used for therapeutic effect in Unani and Ayurvedic System of Medicine. It has been reported to have many therapeutic activities like antifungal, antimicrobial, antioxidant, hepatoprotective and cardioprotective properties. It is also useful in the management of insomnia and CNS disorders. The present study aims towards the evaluation of the parameters involved in the determination of the quality and purity of Nardostachys jatamansi rhizome and its standardization. Methods: Organoleptic characters, extractive values, ash values, phyto-chemical analysis, TLC, fluorescence analysis and HPLC profile etc. were the parameters used for the standardisation of the test drug. Result: Total ash values, water and alcohol soluble extractive values, moisture contents and volatile oil percentage was found to be 7.08%, 3.54%, 2.5%, 8.6% and 02% respectively. TLC profile of N. jatamansi shows 04, 08 and 10 spots in UV short and long wavelength and exposure to iodine vapours respectively. The HPLC pattern shows 34 peaks and the peak no. 01 and 08 are major peaks having area concentration and retention time as 25.974% at 2.8 min. and 29.967% at 4.399 min. respectively. Conclusion: The study will provide referential information for the good quality, purity and identification for the future batches of Nardostachys jatamansi.