Objective: The present study investigated the sub acute toxicity of the ethyl acetate fraction of asam kandis (Garcinia cowa Roxb) Rinds in mice. Material and Methods: Sub acute toxicity study was carried out by giving orally at dose 500, 1000 dan 2000 mg / kgBW extract to five mice at 21 days. Animals were observed individually for any clinical signs of toxicity or mortality for 14 days. Measured parameters were SGPT levels, serum creatinine levels, weight ratio of liver and kidney. Extract was given orally at dose 500, 1000 and 2000 mg/kgBW for 21 days. Observations were done on day 8th, 15th and 22th using blood serum, liver and kidneys of mice. Data were analyzed by using two-way ANOVA followed by Duncan’s Multiple Range Test. Results: The ethyl acetate fraction of G. cowa at doses 500, 1000 and 2000 mg/kgBW gave significant effect on increasing SGPT levels and decreasing levels of serum creatinine (p <0.05). The length of treatment gave significant effect on decreasing levels of serum creatinine, weight ratio of liver and kidney (p <0.05). Conclusion: The dosage of the ethyl acetate fraction of asam kandis rinds provides significant effect on the SGPT and serum creatinine levels of male white mice. The duration of administration of ethyl acetate fraction of asam kandis rinds provides significant effect on serum creatinine levels, the weight ratio of liver and kidney organ of male white mice.