Determination of Rubraxanthone in the Latex of Asam Kandis (Garcinia cowa Roxb) by Reverse Phase High Performance Liquid Chromatography.
Context: Rubraxanthone is a major compound found in Garcinia cowa Roxb which has various biological activities. This compound is likely to be responsible for the pharmacological activities of this plant. The latex of this plant was one of the source of this compound. To prevent counterfeiting, it is essential to develop a method of analysis to determine the levels of these compounds in the latex of G. cowa. Aims: To develop and validated a reverse phase-high performance liquid chromatography (RP-HPLC) technique for determination of rubraxanthone in the latex of G. cowa. Settings and Design: RP-HPLC analysis. Methods and Material: The sample was powdered and dissolve in methanol and then subjected to Reverse Phase High Performace Liquid Chromatoraphy (RP-HPLC). Separation was carried out in a reversed-phase column Shimadzu Shimp-pack VP–ODS (4.6 x 250 mm). The elution was performed with isocratic solvent using formic acid 0.4 % v/v in methanol (15:85) with a flow rate 1 ml/minute. The solvents used for the mobile phase were filtered through membrane filter (0.45 mm pore size) and degassed before use. Total running time was 20 minutes and the sample injection volume of injection was 20 ml. While the wavelength of the UV-VIS detector was set at 243.2 nm. Results: The detection and the quantitation limits of rubraxanthone were 1.119 mg/mL and 3.731 μg/mL, respectively. A regression analysis was performed, with the observation of good linearity (r = 0.998). The values obtained for precision and accuracy determination are in agreement with ICH guidelines. It was found that rubraxanthone in dichloromethane extract of latex G. cowa was 56.56%. Conclusions: The results demonstrated that the developed method is a reliable HPLC technique for determination of rubraxanthone in the latex of G. cowa.