Generation of Multinucleated Giant Cells due to Leishmania (V.) braziliensis Infection

Background: Multinucleated giant cells were first described by Langhans(MGCs), these MGCs have a role in innate immunity that includes extracellular matrix remodeling associated with granuloma formation; another function of them is to participate in the removal of cellular debris in apoptosis during certain infections. Objective: Generate multinucleated giant cells due to Leishmania (V.) braziliensis infection. Methods: For infection macrophage/parasite ratio of 1 to 10 using the RAW 264.7-line (5 x10⁵/mL) and Leishmania (V.) braziliensis metacyclic promastigotes (50 x105/mL) was a strain maintained in culture Schneider liquid medium, supplemented with 20% fetal bovine serum and 10 000 U/10 mg/mL penicillin streptomycin at pH 7 and 25 °C in the laboratory. Incubation was continued for 4 days, and microscopic observation at 1000X was performed at 24 hours and 96 hours, respectively. Results: In our study an MGC conversion was observed at 96 hours (50% ± 28.2). Conclusions: In vitro MGC model could be used to study the physiopathology of MGC generation by Leishmania (V.) braziliensis infection.