<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Boualem HARFI</style></author><author><style face="normal" font="default" size="100%">Lakhdar KHELIFI</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Datura sp.</style></keyword><keyword><style  face="normal" font="default" size="100%">Etiolation</style></keyword><keyword><style  face="normal" font="default" size="100%">Gas chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">Hairy roots</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyoscyamine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">991-995</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Hyoscyamine, because of its medicinal properties, is an important tropane alkaloid. In order to implement a biotechnological process for its production, hairy roots of&lt;em&gt; Datura &lt;/em&gt;species resulting from genetic transformation by &lt;em&gt;Agrobacterium rhizogenes&lt;/em&gt; A4 strain have been the subject of this work. In the &lt;em&gt;in vitro &lt;/em&gt;alkaloids production programs, optimization of the transformation is a crucial step for obtaining a large number of root lines allowing the selection of efficient lines. &lt;strong&gt;Methods:&lt;/strong&gt; The conditions for hairy roots induction were explored by studying the effect of &lt;em&gt;Datura&lt;/em&gt; sp. &lt;em&gt;in vitro&lt;/em&gt; seedling etiolation on the genetic transformation. The first step was the establishment of &lt;em&gt;Datura&lt;/em&gt; &lt;em&gt;in vitro&lt;/em&gt; plantlet cultures followed by the hairy roots induction with A4 strain of &lt;em&gt;A. rhizogenes&lt;/em&gt;. The confirmation of the genetic transformation was performed by polymerase chain reaction (PCR) analysis of rolB gene in the roots. After hyoscyamine extraction, it was analyzed (quantitatively and qualitatively) by gas chromatography. Results: 343 root lines were obtained in total, 96 root lines from non-etiolated &lt;em&gt;Datura in vitro&lt;/em&gt; seedlings and 247 root lines from etiolated &lt;em&gt;in vitro&lt;/em&gt; plantlets. After the selection of six transgenic root lines, tow root lines from each species (&lt;em&gt;D. tramonium, D. tatula &lt;/em&gt;and&lt;em&gt; D. innoxia&lt;/em&gt;), one from non-etiolated &lt;em&gt;in vitro &lt;/em&gt;plantlet and one from etiolated &lt;em&gt;in vitro&lt;/em&gt; plantlet, the most hyoscyamine content was 8.43 mg/g D.W. obtained with &lt;em&gt;D. tatula&lt;/em&gt; etiolated &lt;em&gt;in vitro&lt;/em&gt; seedling. &lt;strong&gt;Conclusion: &lt;/strong&gt;The etiolated&lt;em&gt; in vitro&lt;/em&gt; plantlets seem more favorable to hairy roots induction.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">991</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Boualem HARFI&lt;sup&gt;1,2,&lt;/sup&gt;*, Lakhdar KHELIFI&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Biotechnology Research Center (C.R.Bt), Constantine, ALGERIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;National Higher School of Agronomy (ENSA – El-Harrach), Algiers, ALGERIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mohamed Saleem Thattakudian Sheik Uduman</style></author><author><style face="normal" font="default" size="100%">Prema Rathinam</style></author><author><style face="normal" font="default" size="100%">Yogendrachari Karuru</style></author><author><style face="normal" font="default" size="100%">Gangadhar Obili</style></author><author><style face="normal" font="default" size="100%">Gopinath Chakka</style></author><author><style face="normal" font="default" size="100%">Ashok Kumar Janakiraman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GC-MS Analysis of Ethyl Acetate Extract of Whole Plant of Rostellularia diffusa</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioactive compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Gas chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">phytosterol.</style></keyword><keyword><style  face="normal" font="default" size="100%">Rostellularia diffusa</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">70-72</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; To identify the various phyto constituents present in the unexplored plant &lt;em&gt;Rostellularia diffusa&lt;/em&gt; (Willd.) by using gas chromatography and mass spectrometry. &lt;strong&gt;Methods:&lt;/strong&gt; The whole plant of &lt;em&gt;Rostellularia diffusa &lt;/em&gt;was extracted with ethyl acetate at room temperature for 72 h. The concentrated extract was subjected to GCMS analysis to detect the phyto constituents. &lt;strong&gt;Results:&lt;/strong&gt; Totally 40 compounds were identified and the chromatograph showed 40 peaks with 40 individual compounds. The major constituents were identified in the extract were 16-Hentriacontanone (22.59%), Hexadecanoic acid (11.23%), Stigmast-5-en-3-ol (6.78%), 9-Octadecenoic acid (6.30%) and many other compounds were identified as low level. This preliminary study gives an idea to isolate the major active constituents present in the plant and also helps to develop potential pharmacologically active compounds&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">70</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Mohamed Saleem Thattakudian Sheik Uduman&lt;sup&gt;1*&lt;/sup&gt;, Prema Rathinam&lt;sup&gt;1&lt;/sup&gt;, Yogendrachari Karuru&lt;sup&gt;1&lt;/sup&gt;, Gangadhar Obili&lt;sup&gt;1&lt;/sup&gt;, Gopinath Chakka&lt;sup&gt;1&lt;/sup&gt;, Ashok Kumar Janakiraman&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Annamacharya College of Pharamcy, Rajampet, YSR Kadapa, A.P, INDIA.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur, MALAYSIA.&lt;/p&gt;
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