<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tutik Sri Wahyuni</style></author><author><style face="normal" font="default" size="100%">Adita Ayu Permanasari</style></author><author><style face="normal" font="default" size="100%">Lidya Tumewu</style></author><author><style face="normal" font="default" size="100%">Aty Widyawaruyanti</style></author><author><style face="normal" font="default" size="100%">Achmad Fuad Hafid</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Qualitative and Quantitative Analysis of 70% Ethanol Extract from Ruta angustifolia for Developing Anti-Hepatitis C Agents</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Hepatitis C Virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Infectious disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Ruta angustifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">Rutin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">682-687</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Medicinal plants are potential sources for drug candidates. It possesses with various metabolites which have many pharmacology effects. &lt;em&gt;Ruta angustifolia&lt;/em&gt; is one of medicinal plants that has been used traditionally for liver disease. Previous study it has been demonstrated to inhibit hepatitis C virus under in vitro cell culture. It decreased protein NS3 level and gave synergistic effect in combination with simeprevir and telaprevir. This plant provides a prospective candidate to develop as anti-HCV Objective: This study evaluates the phytochemistry screening for qualitative assay and determine the concentration of rutin as marker compound for developing &lt;em&gt;R. angustifolia&lt;/em&gt; extract as anti-HCV agent.&lt;strong&gt; Materials and Methods:&lt;/strong&gt; &lt;em&gt;R. angustifolia&lt;/em&gt; leaves were extracted with 70% of ethanol. Extract and rutin were analysis their anti-HCV activity by in vitro culture cells of Huh7it. The concentration of rutin was determine by TLC densitometry. &lt;strong&gt;Results:&lt;/strong&gt; The 70% ethanol extract of &lt;em&gt;R. angustifolia &lt;/em&gt;dan rutin exhibit anti-HCV activities with IC&lt;sub&gt;50&lt;/sub&gt; value of 2.9 ± 0.8 μg/ml and 28.1 ± 5.6 μg/ml, respectively. Screening phytochemistry demonstrated to contain flavonoid, terpenoid, alkaloid and polyphenols. TLC densitometry analysis yield the concentration of rutin in extract 0.06 %. &lt;strong&gt;Conclusion:&lt;/strong&gt; Extract of 70% ethanol of &lt;em&gt;R. angustifolia &lt;/em&gt;has a potential anti-HCV activity. Extract of &lt;em&gt;R. angustifolia&lt;/em&gt; may provide a good candidate for developing anti-HCV agents.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">682</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Tutik Sri Wahyuni&lt;sup&gt;1,2,&lt;/sup&gt;*, Adita Ayu Permanasari&lt;sup&gt;2&lt;/sup&gt;, Lidya Tumewu&lt;sup&gt;2&lt;/sup&gt;, Aty Widyawaruyanti&lt;sup&gt;1,2&lt;/sup&gt;, Achmad Fuad Hafid&lt;sup&gt;1,2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Center of Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gaurav Mahesh Doshi</style></author><author><style face="normal" font="default" size="100%">Manjushree kundalik Pawar</style></author><author><style face="normal" font="default" size="100%">Kajal Haribhai Chavda</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Delayed-Type Hypersensitivity response</style></keyword><keyword><style  face="normal" font="default" size="100%">Fourier Transform Infrared Spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Haemagglutination Antibody Titre</style></keyword><keyword><style  face="normal" font="default" size="100%">High performance Thin layer Chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">MCF-7.</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercetin</style></keyword><keyword><style  face="normal" font="default" size="100%">Rutin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Oct 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">476-482</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Albiza lebbeck&lt;/em&gt; leaves have been well known for its ethnopharmacological prospects.&lt;strong&gt; Objective:&lt;/strong&gt; The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and &lt;em&gt;in vitro&lt;/em&gt; cell lines in correlation to analytical studies. &lt;strong&gt;Methods:&lt;/strong&gt; The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for &lt;em&gt;in-vitro&lt;/em&gt; cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. &lt;strong&gt;Results:&lt;/strong&gt; No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 &amp;plusmn; 102.78, 597.33 &amp;plusmn; 85.35, 426.66 &amp;plusmn; 53.98) and DTH Delayed- Type Hypersensitivity reaction (0.225&amp;plusmn;0.01, 0.21 &amp;plusmn; 0.01, 0.23 &amp;plusmn; 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5&amp;times;10&lt;sup&gt;9&lt;/sup&gt;) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on &lt;em&gt;in-vitro&lt;/em&gt; cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 &amp;mu;g/ml, 20 &amp;mu;g/ml, 40 &amp;mu;g/ml, 80 &amp;mu;g/ml.&lt;strong&gt; Conclusion:&lt;/strong&gt; Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">476</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Gaurav Mahesh Doshi&lt;sup&gt;1*&lt;/sup&gt;, Manjushree kundalik Pawar&lt;sup&gt;2&lt;/sup&gt;, Kajal Haribhai Chavda&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Vivekanand Education Society&amp;rsquo;s College of Pharmacy, Chembur (E), Mumbai-400074, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Quality Assurance, Vivekanand Education Society&amp;rsquo;s College of Pharmacy, Chembur (E), Mumbai-400074, INDIA.&lt;/p&gt;
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