<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ayodeji Oluwabunmi Oriola</style></author><author><style face="normal" font="default" size="100%">Pallab Kar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Zinc Oxide Nanoparticle Green Synthesis Using Black Cumin Seed Aqueous Extract: Its Characterization and in vitro Anti- Hyperglycaemic Properties</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-Hyperglycaemia</style></keyword><keyword><style  face="normal" font="default" size="100%">Black cumin</style></keyword><keyword><style  face="normal" font="default" size="100%">ZnO Nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">α-amylase</style></keyword><keyword><style  face="normal" font="default" size="100%">α-glucosidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">434-437</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; α-Amylase and α-glucosidase are carbohydrate metabolizing enzymes that are known to be involved in postprandial hyperglycaemia in diabetic patients. &lt;strong&gt;Objective:&lt;/strong&gt; In a bid to source potent inhibitors of these enzymes, the study synthesized zinc oxide nanoparticles and evaluated the in vitro anti-hyperglycaemic activity.&lt;strong&gt; Materials and Methods:&lt;/strong&gt; The seed aqueous extract of black Cumin (BC) was used as a capping and/or reducing agent to synthesize ZnO NPs from zinc acetate precursor. The BC-ZnONPs were characterized by microscopy (FESEM and HRTEM) and spectroscopy (UV-Vis and EDX) methods. &lt;em&gt;In vitro &lt;/em&gt;anti-hyperglycaemic evaluation was based on α-amylase and α-glucosidase inhibition assays. &lt;strong&gt;Results:&lt;/strong&gt; The BC-ZnONPs showed a spherical-to-cubical shape with a 10-50 nm size range. The UV-Vis absorption peaks at 387 and 415 nm suggest the formation of biogenic ZnO NPs. The EDX spectrum revealed 68.92% and 27.49% weight compositions of Zn and O, respectively, to further substantiate ZnO nanoparticle synthesis. The BC-ZnONPs showed notable anti-hyperglycaemic properties with IC&lt;sub&gt;50&lt;/sub&gt; of 87.72±5.13 and 124.21±15.20 μg/mL against α-amylase and α-glucosidase, respectively.&lt;strong&gt; Conclusion: &lt;/strong&gt;Black Cumin seed extract was a useful biogenic material for synthesizing ZnO NPs. The BCZnONPs showed promising anti-hyperglycaemic properties based on the notable inhibitory activities against α-amylase and α-glucosidase enzymes. Future work may include evaluating the synergistic effects of black Cumin metabolites and ZnONPs, as well as determining the in vivo toxicity profile for safety considerations.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">434</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ayodeji Oluwabunmi Oriola&lt;sup&gt;1*&lt;/sup&gt;, Pallab Kar&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemical and Physical Sciences, Walter Sisulu University, Nelson Mandela Drive, P/ Bag X1, Mthatha 5117, SOUTH AFRICA&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;African Medicinal Flora and Fauna Research Niche Area, Walter Sisulu University, Nelson Mandela Drive, P/Bag X1, Mthatha 5117, SOUTH AFRICA&lt;/p&gt;
</style></auth-address></record></records></xml>