<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bhairav Kumar Pathak</style></author><author><style face="normal" font="default" size="100%">Kamlesh M. Palandurkar</style></author><author><style face="normal" font="default" size="100%">Meenakshi Singh</style></author><author><style face="normal" font="default" size="100%">Anshuman Trigunayat</style></author><author><style face="normal" font="default" size="100%">Amit Singh</style></author><author><style face="normal" font="default" size="100%">Reena Giri</style></author><author><style face="normal" font="default" size="100%">Kiran Rajendra Giri</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Evaluation of In vivo Analgesic and Anti-inflammatory Activity of Oroxyulum indicum, Baicalein, Chrysin with Phytochemical Analysis and Molecular Docking Study</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Baicalein</style></keyword><keyword><style  face="normal" font="default" size="100%">Chrysine</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Oroxylum indicum</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF alpha.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">811-822</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background&lt;/strong&gt;: &lt;em&gt;Oroxyulum indicum &lt;/em&gt;(OIE) is a native medicinal plant that has been widely employed in Ayurvedic medicine for thousands of years. Though studies have been published citing the analgesic and anti-inflammatory activity of &lt;em&gt;Oroxyulum indicum&lt;/em&gt; and chrysin and Baicalein, there has been no comparative study comparing their activittes and confirming them with molecular docking results. Molecular docking study of two phytochemicals Chrysin (PubChem CID 5281607) and Baicalein (PubChem CID 5281605) into the active sites of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Evaluation and validation of Anti-inflammatory and Analgesic effects of a methanolic extract of the stem bark of Oroxylum indicum and its two constituents Chrysin and Baicalein in Charles foster rats with analysis of the phytoconstituent of &lt;em&gt;Oroxyulum indicum &lt;/em&gt;through HRMS analysis. &lt;strong&gt;Methodology: &lt;/strong&gt;UHPLC-HRMS/MS analyses were performed on a Dionex Ultimate 3000 RS Series UHPLC system combined with a Q Exactive Plus High-Resolution Accurate Mass Spectrometry System. Hot plate and Tal flick model are used for screening of analgesic activity. TNF-alpha and IL-6 inflammatory markers were examined. Carrageenan model is used for antiinflammatory analysis. &lt;strong&gt;Result: &lt;/strong&gt;Interesting results has been obtained in the docking studies of Chrysin and Baicalein with COX-1 (PDB ID: 1EQG). The hydrogen bond interaction established between the Chrysin and Baicalein with the important amino acid, includes Arg 120, Tyr 355, Ser 530, Met 522 (Figure 1). The binding free energy of the Chrysin and Baicalein with target COX-1 was found to be -7.88 and -7.26 Kcal/mol. &lt;strong&gt;Conclusion:&lt;/strong&gt; There is marked reduction in the TNF Alpha expression in the OIE group which is followed by Baicalein and Chrysine. The Baicalein group shows the most marked cumulative increase in reaction time for tail flick among all the groups of the intervention group followed by Chrysine and OIE.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">811</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Bhairav Kumar Pathak&lt;sup&gt;1&lt;/sup&gt;, Kamlesh M. Palandurkar&lt;sup&gt;2&lt;/sup&gt;, Meenakshi Singh&lt;sup&gt;3&lt;/sup&gt;, Anshuman Trigunayat&lt;sup&gt;4&lt;/sup&gt;, Amit Singh&lt;sup&gt;5&lt;/sup&gt;, Reena Giri&lt;sup&gt;6&lt;/sup&gt;, Kiran Rajendra Giri&lt;sup&gt;7,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Junior Resident, Department of Pharmacology, IMS, BHU, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Associate Professor, Department of Biochemistry, IMS, BHU, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Assistant Professor, Medicinal Chemistry Department, Faculty of Ayurveda, IMS, BHU, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Professor, Department of Pharmacology, IMS, BHU, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Professor, Department of Pharmacology, IMS, BHU, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Professor, Department of Pharmacology, GMC, Akola, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Associate Professor, Department of Pharmacology, IMS, BHU, INDIA.&lt;/p&gt;
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