<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rangki Astiani</style></author><author><style face="normal" font="default" size="100%">Mohamad Sadikin</style></author><author><style face="normal" font="default" size="100%">Aprilita Rinayanti</style></author><author><style face="normal" font="default" size="100%">Wawaimuli Arozal</style></author><author><style face="normal" font="default" size="100%">Ani Retno Prijanti</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author><author><style face="normal" font="default" size="100%">Firdayani Firdayani</style></author><author><style face="normal" font="default" size="100%">Piter Piter</style></author><author><style face="normal" font="default" size="100%">Guntoro Halim</style></author><author><style face="normal" font="default" size="100%">Franciscus D. Suyatna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Study of Triterpene Saponin Compounds from Centella asitica as Renin Inhibitor with Pharmacophore Modeling, Molecular Docking and In-vitro Evaluation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive.</style></keyword><keyword><style  face="normal" font="default" size="100%">Asiaticoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Centella asiatica</style></keyword><keyword><style  face="normal" font="default" size="100%">In-vitro</style></keyword><keyword><style  face="normal" font="default" size="100%">Madecasoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Renin inhibitor</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">57-63</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Hypertension is a silent killer that causes kidney, heart, and stroke damage if not handled properly. In Indonesia, the prevalence of the population with high blood pressure is 34.11% with women 36.85% higher than men 31.34%, this shows a fairly high value so that special attention is needed on hypertension therapy. It is known that currently there are 6 types of pharmacological therapy for hypertension and one of the newest is the renin inhibitor class (Aliskiren). Indonesia has diverse natural wealth in the form of flora and fauna, with a wealth of more than 30,000 types of medicinal plants with 9500 potential herbal medicines that have not been utilized optimally, with the largest exporter of herbal medicines in the world. &lt;em&gt;Centella asiatica&lt;/em&gt; plants containing triterpenoid saponins have high renin inhibitor activity, namely the content of Asiaticoside and Madecasoside. The research method was carried out &lt;em&gt;in silico&lt;/em&gt; using molecular simulation and &lt;em&gt;in vitro &lt;/em&gt;with fluorometry (328/552 nm) to test the activity of asiaticoside and madecasoside compounds as well as a mixture of asiaticoside and madecasoside in &lt;em&gt;Centella asiatica&lt;/em&gt; plants. This is supported by the docking outcome. The docking results show that madecososide compounds have a gibbs energy close to the positive control aleskiren (-8.356 kcal/mol) and aleskiren (-9.44 kcal/mol). The experiment results showed that the triterpenoid saponin compound (madecassoside) contained an IC value of 0.71, at a concentration of 5 μg/μl, and absorbance of 1.35 A in the first minute. The strongest renin inhibition was Madecasoside compound with a concentration of 5 μg/μl with an average value of fluorescent adsorption and an average percent inhibition of 135% with the best renin inhibition at Madecasoside 5 ug/ul the first minute with absorbance values 1.19 A. Finally, the &lt;em&gt;in silico&lt;/em&gt; result corresponded to the &lt;em&gt;in vitro&lt;/em&gt; experiment. &lt;em&gt;Centella asiatica&lt;/em&gt; plants have renin inhibitor activity as antihypertensive, especially in secondary metabolites of triterpene saponins with pure madecasoside compounds compared with aliskiren as a renin inhibitor. So that the compound madecasoside has renin inhibitor activity as an antihypertensive.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><section><style face="normal" font="default" size="100%">57</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Rangki Astiani&lt;sup&gt;1,3,*&lt;/sup&gt;, Mohamad Sadikin&lt;sup&gt;1,2&lt;/sup&gt;, Aprilita Rinayanti&lt;sup&gt;4&lt;/sup&gt;, Wawaimuli Arozal&lt;sup&gt;1,5&lt;/sup&gt;, Ani Retno Prijanti&lt;sup&gt;1,2&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;1,6,7&lt;/sup&gt;, Firdayani Firdayani&lt;sup&gt;8&lt;/sup&gt;, Piter Piter&lt;sup&gt;3&lt;/sup&gt;, Guntoro Halim&lt;sup&gt;3&lt;/sup&gt;, Franciscus D. Suyatna&lt;sup&gt;1,5&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia 10320, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biology Molecular and Biochemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10320, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Pharmacy, Universitas 17 Agustus 1945 Jakarta, Indonesia 14350, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacy Faculty of Health Sciences, Universitas Esa Unggul, Jakarta 11510, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Bioinformatics Core Facilities - IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Technology Assesment and Application Agency (BPPT), Indonesian Science and Technology Research Center, INDONESIA.&lt;/p&gt;
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