<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dian Pratiwi</style></author><author><style face="normal" font="default" size="100%">Silmi Mariya</style></author><author><style face="normal" font="default" size="100%">Raendi Rayendra</style></author><author><style face="normal" font="default" size="100%">Agus Setiyono</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Analysis and Pro-Melanogenic Activity of Nigella sativa Extract in B16F10 Cells: A Natural Candidate for Vitiligo Treatment</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">B16F10</style></keyword><keyword><style  face="normal" font="default" size="100%">Melanogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Nigella sativa</style></keyword><keyword><style  face="normal" font="default" size="100%">Thymoquinone</style></keyword><keyword><style  face="normal" font="default" size="100%">Tyrosinase</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitiligo</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">307-313</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Vitiligo is a chronic depigmentation disorder caused by the selective destruction of melanocytes, with limited effective treatment options, particularly in resource-limited regions. &lt;em&gt;Nigella sativa &lt;/em&gt;(black cumin seed, BCS) has traditionally been used for various skin ailments, including pigmentation disorders. &lt;strong&gt;Objectives: &lt;/strong&gt;This study aimed to evaluate the pro-melanogenic activity of a 96% ethanol extract of Indonesian BCS (EE BCS) in B16F10 cells. &lt;strong&gt;Methods:&lt;/strong&gt; Phytochemical profiling was performed using gas chromatography–mass spectrometry (GC-MS), and thymoquinone (TQ) content was quantified by highperformance liquid chromatography (HPLC). Cell viability (MTT assay), tyrosinase activity (L-DOPA assay), and melanin content were measured. &lt;strong&gt;Results:&lt;/strong&gt; GC-MS identified 9,12-octadecadienoic acid methyl ester (E,E) as the predominant compound (45.88%), while HPLC confirmed a relatively low TQ concentration (0.04%). EE BCS maintained &amp;gt;90% cell viability at concentrations up to 12.50 ppm and exhibited an IC50 of 56.41 ppm. Tyrosinase activity significantly increased at 6.25 ppm (136.40%; &lt;em&gt;p&lt;/em&gt; &amp;lt; 0.05) and 12.50 ppm (228.10%; &lt;em&gt;p&lt;/em&gt; &amp;lt; 0.01), accompanied by a significant elevation in melanin content (226.00%; &lt;em&gt;p&lt;/em&gt; &amp;lt; 0.05 and 266.90%; &lt;em&gt;p&lt;/em&gt; &amp;lt; 0.01 respectively). &lt;strong&gt;Conclusion: &lt;/strong&gt;EE BCS can effectively promote melanogenesis, despite containing low levels of thymoquinone, potentially through synergistic actions of its phytochemical constituents. Given its region-specific phytochemical richness, Indonesian &lt;em&gt;N. sativa&lt;/em&gt; extract holds promise as a natural therapeutic candidate for vitiligo. Further &lt;em&gt;in-vivo&lt;/em&gt; and clinical validation is warranted.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">307</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Dian Pratiwi&lt;sup&gt;1,*&lt;/sup&gt;, Silmi Mariya&lt;sup&gt;2&lt;/sup&gt;, Raendi Rayendra&lt;sup&gt;3&lt;/sup&gt;, Agus Setiyono&lt;sup&gt;4&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Animal Biomedical Sciences, School of Veterinary Medicine and Biomedicine, IPB University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Primate Animal Study Center, IPB University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Medicine, Syarif Hidayatullah Islamic University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Pathology Division, School of Veterinary Medicine and Biomedicine, IPB University, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dian Ardiana</style></author><author><style face="normal" font="default" size="100%">Lestari Dewi</style></author><author><style face="normal" font="default" size="100%">Renata Prameswari</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In Silico Study of Naringenin as Melanogenesis Inducer in Vitiligo</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Mangrove</style></keyword><keyword><style  face="normal" font="default" size="100%">Naringenin</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizophora mucronata</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitiligo</style></keyword><keyword><style  face="normal" font="default" size="100%">WNT</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">847-857</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Vitiligo is a pigmentation disorder characterized by loss of skin color (depigmentation) due to melanocyte dysfunction and loss. Melanocytes produce melanin pigment through a melanogenesis process. Melanocyte survival and melanogenesis process are influenced by Microphthalmia Associated- Transcription Factor (MITF) and several proteins, including WNT, β-catenin, tyrosinase, Tyrosinase- Related Protein-1 (TRP1), and Tyrosinase-Related Protein-2 (TRP2). The current therapy for vitiligo is still unsatisfactory. Naringenin is one of Rhizophora mucronata compound, one type of mangrove plant often found in the eastern coastal area of Surabaya City. Objective: To investigate the naringenin’s potency in melanogenesis and to predict the pharmacokinetics or toxicity of naringenin by in silico study. &lt;strong&gt;Methods:&lt;/strong&gt; This is a computational study using a molecular docking method to observe the interaction of naringenin with WNT, β-catenin, MITF, tyrosinase, TRP-1, and TRP-2 proteins. Pharmacokinetic or toxicity prediction of naringenin using the pkCSM method. Psoralen was used as a control.&lt;strong&gt; Results&lt;/strong&gt;: Naringenin binds to all these proteins in the same region as psoralen, indicating that naringenin can stimulate melanogenesis. Naringenin has lower binding energy than psoralen on all proteins (except β-catenin), indicating that naringenin's interaction with these proteins is stronger than psoralen. Pharmacokinetic and toxicity predictions show that naringenin has good absorption or permeation, is not mutagenic, is not hepatotoxic, and does not cause skin sensitization. &lt;strong&gt;Conclusion: &lt;/strong&gt;This computational study concludes that naringenin has melanogenesis inducer potency and good pharmacokinetics.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Research Article </style></work-type><section><style face="normal" font="default" size="100%">847</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Dian Ardiana&lt;sup&gt;1,*&lt;/sup&gt;, Lestari Dewi&lt;sup&gt;2&lt;/sup&gt;, Renata Prameswari&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Dermatology and Venereology, Faculty of Medicine, Hang Tuah University, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Hang Tuah University, Surabaya, INDONESIA&lt;/p&gt;
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