<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Annysa Ellycornia Silvyana</style></author><author><style face="normal" font="default" size="100%">Ratika Rahmasari</style></author><author><style face="normal" font="default" size="100%">Berna Elya</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Azadirachta indica Hexane Extract: Potent Antibacterial Activity Against Propionibacterium acne and Identification of its Chemicals Content</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-Propionibacterium acne</style></keyword><keyword><style  face="normal" font="default" size="100%">Azadirachta indica</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemical content.</style></keyword><keyword><style  face="normal" font="default" size="100%">Hexane extract</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">489-496</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Acne is a skin surface disease that appears when the excessive fat deposits clogged the skin pores, causes the growth of acne-causing bacteria and stimulates inflammation.&lt;em&gt; Propionibacterium acnes&lt;/em&gt; is one of common acne-causing bacteria which usually manage by synthetic chemical-based drug. However, the presence of its long- used side effects pointed the urgent need of new anti &lt;em&gt;P. acne&lt;/em&gt; drug discovery. &lt;em&gt;Azadirachta indica &lt;/em&gt;is a medicinal plant which empirically used as antibacterial. &lt;em&gt;A. indica &lt;/em&gt;leaves has been reported to exhibit activity against&lt;em&gt; P. acne &lt;/em&gt;but limited to ethanol extract. Thus, the evaluation of other extract- and identification of active compound(s) against&lt;em&gt; P. acne&lt;/em&gt; is needed to be explore.&lt;strong&gt; Methods:&lt;/strong&gt; First, the microscopic morphology of &lt;em&gt;A. indica&lt;/em&gt; leaves were observed using Scanning Electron Microscope. The leaves were then extracted sequentially by hexane, ethyl acetate, and methanol solvent using the ultrasonic assisted extraction method, followed by its in vitro anti- &lt;em&gt;P. acne&lt;/em&gt; activity evaluation. The most active extract was further evaluated for its chemical(s) content by LC-MS. &lt;strong&gt;Results: &lt;/strong&gt;Scanning Electron Microscope identified the presence of oxalate in the leaves of &lt;em&gt;A. indica.&lt;/em&gt; Evaluation of the anti-&lt;em&gt;P. acne &lt;/em&gt;activity showed that the hexane extract had highest anti-&lt;em&gt;P. acne &lt;/em&gt;compared to others. Further chemical identification showed that hexane extract contains three steroids, one saturated acids and one phenolic compounds.&lt;strong&gt; Conclusions: &lt;/strong&gt;&lt;em&gt;A. indica&lt;/em&gt; hexane extract leaf is prospective to be developed as an acne antibacterial.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><accession-num><style face="normal" font="default" size="100%">02</style></accession-num><section><style face="normal" font="default" size="100%">489</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Annysa Ellycornia Silvyana&lt;sup&gt;1&lt;/sup&gt;, Ratika Rahmasari&lt;sup&gt;2&lt;/sup&gt;, Berna Elya&lt;sup&gt;1,* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Phytochemical, Faculty of Pharmacy, University of Indonesia, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Microbiology, Faculty of Pharmacy, University of Indonesia, INDONESIA.&lt;/p&gt;
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