<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sasithorn Tandhavadhana</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparison of Colchicine Content in Gloriosa superba L., Root after Pretreatment by Thai Traditional Medicine Methods</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Colchicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Gloriosa superba L.</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Pretreatment methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Thai traditional medicine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">942-948</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; In Thai traditional medical practice, the roots of Gloriosa superba L. must be treated prior to use as Thai herbal remedies. Colchicine is a major active chemical constituent in the roots. It was reported that lethal doses of colchicine were 7-26 mg. There have been two pieces of evidence that indicated 5 different pretreatment methods of Gloriosa superba L., roots. Therefore, this research aimed to compare the amount of colchicine before and after pre-treated by 5 different methods and compare colchicine content after pretreatment between methods. &lt;strong&gt;Methods:&lt;/strong&gt; Provide five different locations of fresh Gloriosa superba L., roots. Each sample divided into 6 groups. The first group would be treated as a control, the second to the sixth group was pre-treated by roasting, burning, boiling, stewing, and grilling which follow the methods from two pieces of evidence. Subsequently, quantitative analysis by High Performance Liquid Chromatography. &lt;strong&gt;Results: &lt;/strong&gt;The amount of colchicine in roots before and after pre-treated by roasting, burning, boiling, stewing, and grilling methods were significantly reduced by 45.61±10.61% (&lt;em&gt;p&lt;/em&gt;=0.000), 37.35±14.15% (&lt;em&gt;p&lt;/em&gt;=0.000), 44.55±8.60% (&lt;em&gt;p&lt;/em&gt;=0.000), 76.40±10.70% (&lt;em&gt;p&lt;/em&gt;=0.000), and 30.35±11.31% (&lt;em&gt;p&lt;/em&gt;=0.000), respectively. The stewing method was the greatest decrease amount of colchicine which significantly different from other methods. (&lt;em&gt;p&lt;/em&gt;=0.000).&lt;strong&gt; Conclusion: &lt;/strong&gt;All 5 methods were significantly reduced amounts of colchicine in the roots of Gloriosa superba L. They were effective methods to pre-treat the roots of Gloriosa superba L. prior to use as Thai herbal remedies.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">942</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sasithorn Tandhavadhana*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Faculty of Science, Ramkhamhaeng University, Bangkok 10240, THAILAND.&lt;/p&gt;
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