<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sumet Kongkiatpaiboon</style></author><author><style face="normal" font="default" size="100%">Piyanuch Rojsanga,</style></author><author><style face="normal" font="default" size="100%">Virote Pattarajinda,</style></author><author><style face="normal" font="default" size="100%">Wandee Gritsanapan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acetylcholinesterase inhibitory activity of didehydrostemofoline, stemofoline alkaloids and extracts from Stemona collinsiae Craib roots</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetylcholinesterase inhibition</style></keyword><keyword><style  face="normal" font="default" size="100%">Didehydrostemofoline</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemofoline</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemona collinsiae</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemonaceae</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">56-59</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Acetylcholinesterase (AChE) inhibition is one of insect toxicity mechanisms found in many plants. &lt;em&gt;Stemona collinsiae&lt;/em&gt; Craib, a traditional insecticidal plant, has been of interest for its AChE inhibitory activity.&lt;strong&gt; Methods:&lt;/strong&gt; Powdered roots of &lt;em&gt;S. collinsiae&lt;/em&gt; were successively extracted with hexane, dichloromethane, and methanol. The contents of major active insecticidal components, didehydrostemofoline and stemofoline alkaloids, were analyzed by HPLC. Anti-AChE activity was evaluated using Ellman&amp;rsquo;s colorimetric method and TLC-bioautography. &lt;strong&gt;Results:&lt;/strong&gt; The contents of didehydrostemofoline and stemofoline alkaloids in the hexane, dichloromethane, and methanol extracts were 3.59 and 0.18, 40.78 and 0.74, and 1.43 and 0.09% w/w, respectively. TLC fingerprints of each extract showed major spots of didehydrostemofoline and stemofoline of which TLC bioassays indicated active AChE inhibitory activity. IC&lt;sub&gt;50&lt;/sub&gt; values on anti-AChE activities of hexane, dichloromethane and methanol extracts were 126.72, 73.78, and &amp;gt;1000 mg/ml, respectively while those of didehydrostemofoline, stemofoline, and standard galanthamine were 131.3, 102.1, and 1.30 mM, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; The AChE inhibitory activity of hexane, dichloromethane and methanol extracts from &lt;em&gt;S. collinsiae&lt;/em&gt; roots and their major alkaloids, didehydrostemofoline and stemofoline, were determined. The data support the traditional utilization of this plant as a natural insecticide.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Acetylcholinesterase inhibition, Didehydrostemofoline, Stemofoline, &lt;em&gt;Stemona collinsiae&lt;/em&gt;, Stemonaceae.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;strong&gt;Sumet Kongkiatpaiboon&lt;sup&gt;a&lt;/sup&gt;, Piyanuch Rojsanga&lt;sup&gt;b&lt;/sup&gt;, Virote Pattarajinda&lt;sup&gt;c&lt;/sup&gt;, Wandee Gritsanapan&lt;sup&gt;a,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;a&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayutthaya Rd, Ratchathewi, Bangkok 10400, Thailand&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;b&lt;/sup&gt;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;c&lt;/sup&gt;Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand&lt;/p&gt;</style></auth-address></record></records></xml>