<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Madhukiran Parvathaneni,</style></author><author><style face="normal" font="default" size="100%">Ganga Rao Battu,</style></author><author><style face="normal" font="default" size="100%">Ravikumar Jangiti,</style></author><author><style face="normal" font="default" size="100%">Keerthana Diyya</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pharmacokinetic study of phyllanthin and hypophyllanthin after oral administration to rats</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">HPLC–PDA</style></keyword><keyword><style  face="normal" font="default" size="100%">hypophyllanthin</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacokinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Phyllanthin</style></keyword><keyword><style  face="normal" font="default" size="100%">Phyllanthus amarus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">18th Feb,2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">124-130</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; The present study was carried out to develop a sensitive and cost effective HPLC method for the determination of bioactive lignans (phyllanthin and hypophyllanthin) and its application in a pharmacokinetic study. &lt;strong&gt;Methods:&lt;/strong&gt; Identification of lignan compounds on C&amp;ndash;18 column was monitored at a range of 199&amp;ndash;400nm using photodiode array detector (PDA) with methanol-water (66:34, v/v) as mobile phase at a flow rate of 1ml/min. Carbamazepine was used as internal standard. &lt;strong&gt;Results:&lt;/strong&gt; From the developed method LOD and LOQ values were found to be 56.14ng/ml and 169.99ng/ml for phyllanthin, and 56.04ng/ml and 169.82ng/ml for hypophyllanthin. The validated RP&amp;ndash;HPLC method herein was applied for pharmacokinetic studies and C&lt;sub&gt;max&lt;/sub&gt; (ng/ml) values for administered three oral doses (2.5, 5 and 10mg/kg) of phyllanthin and hypophyllanthin were 0.28&amp;plusmn;0.06, 0.53&amp;plusmn;0.16, 0.98&amp;plusmn;0.22 and 0.68&amp;plusmn;0.76, 1.35&amp;plusmn;0.23, 2.45&amp;plusmn;0.33, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; In conclusion, developed HPLC&amp;ndash;PDA method effectively determined the phyllanthin and hypophyllanthin in various solvent and plasma samples. This method was successfully applied in conducting their oral pharmacokinetic studies.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Key words:&lt;/strong&gt;&lt;em&gt;Phyllanthus amarus&lt;/em&gt;, phyllanthin, hypophyllanthin, HPLC–PDA, pharmacokinetics.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">124</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Madhukiran Parvathaneni&lt;sup&gt;*,a&lt;/sup&gt;, Ganga Rao Battu&lt;sup&gt;a&lt;/sup&gt;, Ravikumar Jangiti&lt;sup&gt;a&lt;/sup&gt; and Keerthana Diyya&lt;/strong&gt;&lt;sup&gt;&lt;strong&gt;a &lt;/strong&gt;&lt;/sup&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;a&lt;/sup&gt;University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530 003, India.&lt;/p&gt;</style></auth-address></record></records></xml>