<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Swapna Gurrapu</style></author><author><style face="normal" font="default" size="100%">Estari Mamidala</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Antibacterial Activity of Alkaloids Isolated from Leaves of Eclipta alba Against Human Pathogenic Bacteria</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agarwell diffusion</style></keyword><keyword><style  face="normal" font="default" size="100%">Alkaloid</style></keyword><keyword><style  face="normal" font="default" size="100%">Antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Broth micro-dilution assay.</style></keyword><keyword><style  face="normal" font="default" size="100%">Eclipta alba</style></keyword><keyword><style  face="normal" font="default" size="100%">Human pathogenic bacteria</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/PJ-9-4/10.5530pj.2017.4.91</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">573-577</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 &amp;mu;g/ml against all the bacteria. In this study, the &lt;em&gt;S. aureus &lt;/em&gt;and &lt;em&gt;E. coli&lt;/em&gt; are the more susceptible than the other selected human pathogenic bacteria. Based on the observations, &lt;em&gt;E. alba&lt;/em&gt; appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections. &lt;strong&gt;Context:&lt;/strong&gt; Different parts of Eclipta alba crude extracts are used traditionally for the treatment of several diseases of liver, skin and stomach. &lt;strong&gt;Aims:&lt;/strong&gt; To study the &lt;em&gt;in vitro&lt;/em&gt; antimicrobial activity of alkaloids isolated from &lt;em&gt;E. alba&lt;/em&gt; against human pathogenic bacteria. &lt;strong&gt;Settings and Design:&lt;/strong&gt; The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. &lt;strong&gt;Methods and Material:&lt;/strong&gt; The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. Agar-well diffusion and Broth micro-dilution methods were selected to assess the antimicrobial activity. &lt;strong&gt;Statistical analysis used:&lt;/strong&gt; One-way analysis of variance (ANOVA) was used to determine statistical significance. &lt;strong&gt;Results:&lt;/strong&gt; In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 &amp;mu;g/ml against all the bacteria. In this study, the &lt;em&gt;S. aureus&lt;/em&gt; and &lt;em&gt;E. coli&lt;/em&gt; are the more susceptible than the other selected human pathogenic bacteria. &lt;strong&gt;Conclusion:&lt;/strong&gt; Based on the observations, &lt;em&gt;E. alba&lt;/em&gt; appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">573</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Swapna Gurrapu and Estari Mamidala&lt;sup&gt;* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;em&gt;Infectious Diseases Research Lab, Department of Zoology, Kakatiya University, Warangal- 506009, telangana State, INDIA.&lt;/em&gt;&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sunita Shailajan</style></author><author><style face="normal" font="default" size="100%">Sasikumar Menon</style></author><author><style face="normal" font="default" size="100%">Dipti Singh</style></author><author><style face="normal" font="default" size="100%">Gauri Swar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validated analytical RP-HPLC method for quantitation of wedelolactone from Eclipta alba and marketed Ayurvedic formulations</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Eclipta alba</style></keyword><keyword><style  face="normal" font="default" size="100%">Formulations</style></keyword><keyword><style  face="normal" font="default" size="100%">RP-HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Validation</style></keyword><keyword><style  face="normal" font="default" size="100%">Wedelolactone.</style></keyword><keyword><style  face="normal" font="default" size="100%">Whole plant</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">132-139</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Introduction&lt;/strong&gt;: &lt;em&gt;Eclipta alba&lt;/em&gt; Linn. (Asteraceae) is an important ingredient of several Ayurvedic formulations. The monograph on different parts of plant like flowers, leaves, roots are listed in Ayurvedic pharmacopeia of India. The plant is reported to be effective for broad range maladies like inflammation, reproductive problems of females etc. It is also used as a hepatoprotectant, analgesic, antibacterial and antidiabetic agent. Wedelolactone is used as a bioactive marker to establish the quality of the crude drug and its formulations. In the present study, wedelolactone-based standardization of &lt;em&gt;Eclipta alba&lt;/em&gt; and its quantitation from marketed herbal and Ayurvedic formulations has been documented using RP-HPLC. &lt;strong&gt;Methods&lt;/strong&gt;: In the current work, an isocratic method has been developed and validated to quantitate wedelolactone from whole plant of &lt;em&gt;Eclipta alba&lt;/em&gt;. This method is validated as per ICH guidelines and is used to quantitate the content of wedelolactone in polyherbal formulations like Liv52, Geriforte, Mahabhringaraj oil etc. &lt;strong&gt;Results: &lt;/strong&gt;The LOD is found to be 0.5 &amp;mu;g/mL and the LOQ is 1 &amp;mu;g/mL. The linearity range of response is from 5 &amp;mu;g/mL to 100 &amp;mu;g/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; The validated method is found to be simple, sensitive, accurate, rugged and reproducible. This developed method can be recommended for quality assurance and marker-based standardization of polyherbal formulations containing whole plant of &lt;em&gt;Eclipta alba.&lt;/em&gt;&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">132</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sunita Shailajan&lt;sup&gt;1&lt;/sup&gt;, Sasikumar Menon&lt;sup&gt;2&lt;/sup&gt;, Dipti Singh&lt;sup&gt;1&lt;/sup&gt;, Gauri Swar&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai - 400 019, India&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Institute for Advanced Training and Research in Interdisciplinary Science, Plot No. 194, Scheme No. 6, Road No. 15, Sion Koliwada, Sion (East), Mumbai-400 022, INDIA.&lt;/p&gt;
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