<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sreelakshmi Bada Venkatappa Gari</style></author><author><style face="normal" font="default" size="100%">Ramalingam Peraman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Antitubercular</style></keyword><keyword><style  face="normal" font="default" size="100%">HCT-116</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunomodulatory</style></keyword><keyword><style  face="normal" font="default" size="100%">Tinospora sinensis</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF-Alpha</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">8-16</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of &lt;em&gt;Tinospora sinensis&lt;/em&gt; (TS). &lt;strong&gt;Objective: &lt;/strong&gt;natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt; H&lt;sub&gt;37&lt;/sub&gt;Rv (Mtb) by luciferase reporter phage (LPS) assay method.&lt;strong&gt; Results:&lt;/strong&gt; The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (&lt;em&gt;p&lt;/em&gt;&amp;lt;0.05) on HCT-116 cells with the IC&lt;sub&gt;50&lt;/sub&gt; of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC&lt;sub&gt;50&lt;/sub&gt; of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (&lt;em&gt;P&lt;/em&gt;&amp;lt;0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 % at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. &lt;strong&gt;Conclusion: &lt;/strong&gt;Overall, we scientifically support the traditional use&lt;em&gt; Tinospora sinensis&lt;/em&gt; stem in the treatment of cancer and immune diseases.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">8</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sreelakshmi Bada Venkatappa Gari&lt;sup&gt;1,&lt;/sup&gt;*, Ramalingam Peraman&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Research Scholar, Faculty of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Anantapur (JNTUA), Anantapur, Andhra Pradesh 515002, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Professor of Pharmaceutical and Medicinal chemistry RERDS-Centre for Pharmaceutical Research, Raghavendra Institute of Pharmaceutical Education and Research (RIPER)-Autonomous, Anantapur, Andhra Pradesh 515721, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Allan Patrick G. Macabeo</style></author><author><style face="normal" font="default" size="100%">Christopher A. Lee</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Sterols and triterpenes from the non-polar antitubercular fraction of Abutilon indicum</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Abutilon indicum</style></keyword><keyword><style  face="normal" font="default" size="100%">Antitubercular</style></keyword><keyword><style  face="normal" font="default" size="100%">Malvaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Squalene</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol.</style></keyword><keyword><style  face="normal" font="default" size="100%">β-amyrin 3-palmitate</style></keyword><keyword><style  face="normal" font="default" size="100%">β-sitosterol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">26th May 2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">49-52</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;&lt;em&gt;Abutilon indicum Sweet&lt;/em&gt; (Malvaceae) is used in Filipino folk medicine as demulcent, diuretic, sedative, aphrodisiac and antidiabetic remedy. It is also effective in the treatment of leprosy. It is also used as a laxative for patients having hemorrhoids and in the treatment of coughs, puerperal disease, urinary disorders, chronic dysentery and fever. &lt;strong&gt;Methods:&lt;/strong&gt; Air-dried powdered leaves of &lt;em&gt;A. indicum&lt;/em&gt; were initially extracted with DCM-methanol (1:1) to afford a crude extract which was fractionated on a silica gel column to afford four fractions. The first fraction which showed antimycobacterial activity was further chromatographed in a silica gel column to afford bioactive fractions from which four compounds were obtained. The crude extract, fractions and isolated compounds were screened for their inhibitory effect against &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt; H&lt;sub&gt;37&lt;/sub&gt;Rv (ATCC) using the colorimetric Microplate Alamar Blue Assay (MABA) method. &lt;strong&gt;Results: &lt;/strong&gt;The fractions obtained after silica gel chromatography of the crude DCMmethanol (1:1) revealed the first fraction (MIC=64 mg/mL) to exert the highest inhibition against &lt;em&gt;M. tuberculosis&lt;/em&gt; H&lt;sub&gt;37&lt;/sub&gt;Rv. Further separation of this fraction afforded sub-fractions with moderately strong inhibitory activity against the test organism (MIC up to 64 mg/mL). Chromatographic purification of sub-fraction 1 afforded four compounds which were spectroscopically identified as &amp;beta;-amyrin 3-palmitate (1), squalene (2) and a 1:1 mixture of the sterols &amp;beta;-sitosterol (3) and stigmasterol (4). Evaluation of the antimycobacterial activity of 1&amp;ndash;4 showed insignificant inhibitory activity against the test organism (MIC = &amp;gt;128 mg/mL). &lt;strong&gt;Conclusion:&lt;/strong&gt; The present results demonstrate the potential of &lt;em&gt;Abutilon indicum&lt;/em&gt; as a plant source of compounds that may exhibit promising antituberculosis activity. While the known compounds isolated for this plant did not show antimycobacterial activity, the obtained results are considered sufficient reason for further study to isolate the metabolites from &lt;em&gt;A. indicum&lt;/em&gt; responsible for the antitubercular activity.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt;&lt;em&gt;Abutilon indicum&lt;/em&gt;, Malvaceae, antitubercular, squalene, &amp;beta;-amyrin 3-palmitate, &amp;beta;-sitosterol, stigmasterol.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Allan Patrick G. Macabeo&lt;sup&gt;a,b,*&lt;/sup&gt; and Christopher A. Lee&lt;/strong&gt;&lt;sup&gt;&lt;strong&gt;a&lt;/strong&gt;&lt;/sup&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;a&lt;/sup&gt;Organic Synthesis and Phytochemistry Laboratory, Research Center for the Natural and Applied Sciences, University of Santo Tomas, Espana St., Manila 1015, Philippines&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;b&lt;/sup&gt;Institutf&amp;uuml;rOrganischeChemie, Universit&amp;auml;t Regensburg, Universit&amp;auml;tsstrasse 31, 93053 Regensburg, Germany.&lt;/p&gt;</style></auth-address></record></records></xml>