<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sreelakshmi Bada Venkatappa Gari</style></author><author><style face="normal" font="default" size="100%">Ramalingam Peraman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Antitubercular</style></keyword><keyword><style  face="normal" font="default" size="100%">HCT-116</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunomodulatory</style></keyword><keyword><style  face="normal" font="default" size="100%">Tinospora sinensis</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF-Alpha</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">8-16</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of &lt;em&gt;Tinospora sinensis&lt;/em&gt; (TS). &lt;strong&gt;Objective: &lt;/strong&gt;natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt; H&lt;sub&gt;37&lt;/sub&gt;Rv (Mtb) by luciferase reporter phage (LPS) assay method.&lt;strong&gt; Results:&lt;/strong&gt; The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (&lt;em&gt;p&lt;/em&gt;&amp;lt;0.05) on HCT-116 cells with the IC&lt;sub&gt;50&lt;/sub&gt; of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC&lt;sub&gt;50&lt;/sub&gt; of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (&lt;em&gt;P&lt;/em&gt;&amp;lt;0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 % at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. &lt;strong&gt;Conclusion: &lt;/strong&gt;Overall, we scientifically support the traditional use&lt;em&gt; Tinospora sinensis&lt;/em&gt; stem in the treatment of cancer and immune diseases.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">8</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sreelakshmi Bada Venkatappa Gari&lt;sup&gt;1,&lt;/sup&gt;*, Ramalingam Peraman&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Research Scholar, Faculty of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Anantapur (JNTUA), Anantapur, Andhra Pradesh 515002, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Professor of Pharmaceutical and Medicinal chemistry RERDS-Centre for Pharmaceutical Research, Raghavendra Institute of Pharmaceutical Education and Research (RIPER)-Autonomous, Anantapur, Andhra Pradesh 515721, INDIA.&lt;/p&gt;
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