<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kukuh Dwiputra Hernugrahanto</style></author><author><style face="normal" font="default" size="100%">Naufaldy Rifqiaulia Noerda</style></author><author><style face="normal" font="default" size="100%">Jifaldi Afrian Maharaja Dinda Sedar</style></author><author><style face="normal" font="default" size="100%">Lukas Widhiyanto</style></author><author><style face="normal" font="default" size="100%">Dwikora Novembri Utomo</style></author><author><style face="normal" font="default" size="100%">Djoko Santoso</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Natural Cartilage-Derived Scaffolds for 3D Mesenchymal Stem Cell Culture: Promoting Chondrogenesis and Modulating Secretome Composition</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cartilage</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Secretome</style></keyword><keyword><style  face="normal" font="default" size="100%">Stem Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Three-Dimensional</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">1365-1372</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;The regenerative potential of stem cells lies in their secretome, a collection of signaling molecules whose composition is shaped by the cellular microenvironment and external stimuli. To enhance cartilage regeneration, a 3D culture system using a natural cartilage scaffold has been developed to create a more chondrogenic secretome. This study investigates the chondrogenic differentiation of mesenchymal stem cells (MSCs) cultured in a decellularized bovine cartilage scaffold and analyzes the resulting secretome's composition. &lt;strong&gt;Methods: &lt;/strong&gt;This study employed a randomized time series design to examine MSCs chondrogenic differentiation. A control group was cultured in standard 2D conditions, while two experimental groups were cultured in either 2D medium supplemented with chondrocyte differentiation medium (positive control) or a 3D decellularized bovine cartilage scaffold. The study hypothesized that the 3D culture would promote chondrogenesis at least as effectively as the positive control. Key chondrogenic markers were evaluated at various time points.&lt;strong&gt; Results: &lt;/strong&gt;Statistical analysis revealed significant differences in marker expression between the experimental and control groups. SOX-9 and aggrecan were elevated in both experimental groups. The 3D group showed higher RUNX-2 expression and the highest Coll-2 expression at later time points. Additionally, growth factor analysis showed the 3D group had the highest levels of IGF-1 and FGF-2 towards the end of the study. &lt;strong&gt;Conclusion: &lt;/strong&gt;3D culture of MSCs in a bovine cartilage scaffold enhances chondrogenic differentiation and produces a secretome with comparable chondrogenic potential to traditional 2D culture with differentiation medium, suggesting its promise for cartilage regeneration.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1365</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kukuh Dwiputra Hernugrahanto&lt;sup&gt;1,2,3&lt;/sup&gt;, Naufaldy Rifqiaulia Noerda&lt;sup&gt;4&lt;/sup&gt;, Jifaldi Afrian Maharaja Dinda Sedar&lt;sup&gt;2,3&lt;/sup&gt;, Lukas Widhiyanto&lt;sup&gt;2,3&lt;/sup&gt;, Dwikora Novembri Utomo&lt;sup&gt;2,3&lt;/sup&gt;, Djoko Santoso&lt;sup&gt;5,6*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Orthopaedic and Traumatology, Dr Soetomo General Academic Hospital, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Siti Khodijah Hospital, Sidoarjo, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Internal Medicine, Dr Soetomo General Academic Hospital, Surabaya, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yudi Her Oktaviono</style></author><author><style face="normal" font="default" size="100%">Melly Susanti</style></author><author><style face="normal" font="default" size="100%">Achmad Lefi</style></author><author><style face="normal" font="default" size="100%">Ferry Sandra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Human Umbilical Cord Blood-derived Secretome Enhance Endothelial Progenitor Cells Migration on Hyperglycemic Conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Endothelial progenitor cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyperglycemia state</style></keyword><keyword><style  face="normal" font="default" size="100%">Mesenchymal stem cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Migration</style></keyword><keyword><style  face="normal" font="default" size="100%">Secretome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">793-797</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Hyperglycemia state is harmful to body’s homeostasis. Uncontrolled hyperglycemic patients, especially patients with diabetes mellitus have a higher mortality risk of heart disease 2 to 4 times compared to non-hyperglycemic patients. Vascular endothelial impairment always been observed and found as a key feature of hyperglycemia state, which is correlated with reduced numbers and dysfunction of endothelial progenitor cells (EPCs). &lt;strong&gt;Objective: &lt;/strong&gt;This paper aims to investigate the effect of hUCB-MSCs derived secretome treatment on the EPCs migration under hyperglycemia state. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;EPCs were isolated and cultured from peripheral blood samples and cultured for three days. Cultured EPCs were cultivated in 6-well plates until confluence and incubated with high glucose for 5 days, then placed in the modified Boyden chamber at the upper chamber with basal media. The lower chamber was supplemented with basal media and secretome at 2%, 10%, and 20% concentration and VEGF treated group as a control. EPCS migration was evaluated using a Boyden chamber assay. Statistical analysis was performed using SPS 25.0. &lt;strong&gt;Results: &lt;/strong&gt;EPCs migration were significantly higher when hUCB-MSCs-derived secretome was given in high glucose concentrations compared to the and control group (79.80 ± 5.07 vs 51.00 ± 5.15, &lt;em&gt;p&lt;/em&gt;&amp;lt;0.000). This study also showed that hUCB-MSCs-derived secretome increase EPCs migration under high glucose concentrations in a dose-dependent manner (&lt;em&gt;p&lt;/em&gt;&amp;lt;0.05). &lt;strong&gt;Conclusion:&lt;/strong&gt; hUCB-MSCsderived secretome enhances EPCs migration under hyperglycemic state. This result may be of relevance for cell-free and regenerative therapeutic modality for a diabetic patient with coronary artery disease (CAD).&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">793</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Yudi Her Oktaviono&lt;sup&gt;1,&lt;/sup&gt;*, Melly Susanti&lt;sup&gt;1,&lt;/sup&gt;*, Achmad Lefi&lt;sup&gt;1&lt;/sup&gt;, Ferry Sandra&lt;sup&gt;2,3 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga, Prof Moestopo Street 6-8, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Prodia Stem Cell Laboratory, Kramat 7 No. 11 Street, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Universitas Trisakti, Kyai Tapa Street No.260, Jakarta, INDONESIA.&lt;/p&gt;
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