<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Effects of Imperata cylindrica Root Ethanol Extract on the Expression of PKCα, STAT3, and mTOR in A549 Lung Cancer Cell Line</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword><keyword><style  face="normal" font="default" size="100%">mTOR</style></keyword><keyword><style  face="normal" font="default" size="100%">PKCα</style></keyword><keyword><style  face="normal" font="default" size="100%">STAT3</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">284-291</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Imperata cylindrica&lt;/em&gt; or Cogon grass is a medicinal plant that has an anticancer effect. We unravel the cytotoxic effect of Imperata cylindrica root ethanol extract on the expression of PKCα, STAT3 and mTOR in A549 lung cancer cell lines. &lt;strong&gt;Methods: &lt;/strong&gt;The cytotoxic effect tests were carried out on the A549 lung cancer cell line after 48 hours post-treatment using 3- [4, 5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay method to discover the IC&lt;sub&gt;50&lt;/sub&gt; dose, continued with apoptosis tests at doses of 150, 300, and 600 μg/mL of &lt;em&gt;I.cylindrica&lt;/em&gt; root ethanol extract using flowcytometry, as well as measurements of PKCα, STAT3, and mTOR mRNA expressions with the RT-qPCR method and measurements of PKCα, STAT3, and mTOR protein expressions with Western blot method. &lt;strong&gt;Results: &lt;/strong&gt;This study showed that the ethanol extract of &lt;em&gt;I. cylindrica&lt;/em&gt; roots had an IC&lt;sub&gt;50&lt;/sub&gt; of 541 μg/mL (weak cytotoxic) and the IC&lt;sub&gt;50 &lt;/sub&gt;of erlotinib was at 29 μM (moderate cytotoxic). In apoptotic test, the highest average apoptotic results were found in erlotinib group, while ethanol extract doses of 150, 300, and 600 μg/mL gave apoptotic effects on cancer cells higher than negative control group. From RT-qPCR, found that &lt;em&gt;I.cylindrica&lt;/em&gt; significantly inhibit the expression of PKCα, STAT&lt;sub&gt;3&lt;/sub&gt;, and mTOR mRNA and from band scanning using Western Blot showed PKCα, STAT3, and mTOR protein expressions in Imperata root ethanol extract, qualitatively produced thinner bands when compared to the negative control group. &lt;strong&gt;Conclusion:&lt;/strong&gt; This study demonstrated&lt;em&gt; I.cylindrica&lt;/em&gt; root ethanol extract ability inhibiting the expression of PKCα, STAT3 and mTOR genes and proteins in the A549 lung cancer cell line, which can be taken into consideration as a complementary therapy in treatment of lung cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">284</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1,*&lt;/sup&gt;, Eko Fuji Ariyanto&lt;sup&gt;2&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;3&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;4&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&amp;nbsp;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Synergistic Effect of Imperata cylindrica Root Extract and Erlotinib on A549 Lung Cancer Cell Viability</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell viability</style></keyword><keyword><style  face="normal" font="default" size="100%">Erlotinib</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">656-659</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Erlotinib is typically used to treat lung adenocarcinoma and Imperata cylindrica extract has been shown to exert anti-cancer effects in various human cancer cell lines. Therefore, this study evaluated the combined anticancer effects of Imperata cylindrica ethanol root extract and erlotinib on the A549 lung cancer cell line. &lt;strong&gt;Method:&lt;/strong&gt; The A-549 lung cancer cell line was treated with various combinations of 1/2 IC50, 3/8 IC50, 1/4 IC50, and 1/8 IC50 of I.cylindrica root ethanol extract and erlotinib for 48 hours. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, analyzed and interpreted using CompuSyn software and a normalized isobologram curve. &lt;strong&gt;Result:&lt;/strong&gt; Of the sixteen combinations, eleven combinations acted synergistically, four were additive, and one was in the antagonist zone. &lt;strong&gt;Conclusion:&lt;/strong&gt; The combination of I. cylindrica root ethanol extract and erlotinib act synergistically to decrease A549 lung cancer cell viability, therefore they are potential lung cancer therapeutics.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">656</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1&lt;/sup&gt;*, Eko Fuji Ariyanto&lt;sup&gt;2&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;3&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;4&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell viability</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperatorin</style></keyword><keyword><style  face="normal" font="default" size="100%">Sinensetin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">38-46</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Lung cancer remains the leading cause of cancer death worldwide, so research is ongoing to discover new therapeutics, such as plant-derived bioactive compounds. For example, Sinensetin, a plant-derived polymethoxylated flavonoid, and Imperatorin, a natural furanocoumarin, have anti-cancer properties. This study assessed the effects of sinensetin and imperatorin separately and in combination on A-549 lung cancer cell viability. &lt;strong&gt;Method: &lt;/strong&gt;The A-549 lung cancer cell line was treated with sinensetin (60 μM), imperatorin (30 M), or a combination of both compounds (Sin:Imp 30:30 μM; 50:50 μM and 60:30 μM) for 48 hours. Cell viability was then assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and apoptosis was determined using fluorescein isothiocyanate (FITC) Annexin-V/Propidium iodide staining. &lt;strong&gt;Results: &lt;/strong&gt;The combination treatment of Sin:Imp 50:50 and Sin:Imp 60:30 μM reduced cell viability more than the individual treatment of sinensetin and imperatorin, with the lowest cell viability observed for the combination treatment of Sin:Imp 50:50 μM. Likewise, the combination treatment of Sin:Imp 50:50 μM induced the most apoptosis compared to individual treatment. &lt;strong&gt;Conclusion:&lt;/strong&gt; Sinensetin and imperatorin can decrease A-549 lung cancer cell viability and are potent apoptotic inducers, especially when they are used in combination, therefore they are potential lung cancer therapeutics.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><section><style face="normal" font="default" size="100%">38</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1,2,*&lt;/sup&gt;, Eko Fuji Ariyanto&lt;sup&gt;3&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;4&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;5&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Doctoral Program in Medical Science, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ankita Mridha</style></author><author><style face="normal" font="default" size="100%">Priya K Gopal</style></author><author><style face="normal" font="default" size="100%">Santanu Paul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening Data Reveals that Spirogyra triplicata, a Fresh Water Algae Induces Robust Anti-Proliferative Activity Against A549 Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-proliferative</style></keyword><keyword><style  face="normal" font="default" size="100%">GCMS</style></keyword><keyword><style  face="normal" font="default" size="100%">Spirogyra triplicata</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">569-577</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Algae comprise a promising source of novel components with potent therapeutic agents. In particular, algae have been considered as a potential source of new bioactive compounds. The antioxidant data of our previous study with six different algal methanolic extract reveals the presence of high antioxidant, total phenol content and total flavonoid content in &lt;em&gt;Spirogyra triplicata. &lt;/em&gt;Thus, we further focused on screening the anti-proliferative activity of six different green algae on five different cancer cell lines like MCF7, A549, HEPG2, REH, MOLT4.&lt;strong&gt; Methods:&lt;/strong&gt; To fulfill our aim we performed MTT assay for testing anti-proliferative activity and DAPI staining for observing nuclear morphology. We also looked into the metabolomic profiling of &lt;em&gt;Spirogyra triplicata&lt;/em&gt; by GC-MS chemometric study. &lt;strong&gt;Results:&lt;/strong&gt; The result indicates that after 24 hours of treatment with methanolic extract of&lt;em&gt; Spirogyra triplicata &lt;/em&gt;A549 was the most sensitive cell line with IC&lt;sub&gt;50 &lt;/sub&gt;value of 24.07 ± 1.09 μg/ml. Followed by&lt;em&gt; Rhizoclonium fontinale&lt;/em&gt; and &lt;em&gt;Hydrodictyon reticulatum&lt;/em&gt; with IC&lt;sub&gt;50 &lt;/sub&gt;value of 25.97 ± 1.94 μg/ml and 32.50 ± 1.97 μg/ml respectively. The HEPG2 cell line was the second most sensitive cell line against&lt;em&gt; S. triplicata &lt;/em&gt;with IC&lt;sub&gt;50&lt;/sub&gt; value of 30.20 ± 1.45 μg/ml. The MOLT4 cell line was detected as most resistant cell line against the green algal extract in this study. Though the methanolic extracts of six green algae showed maximum to moderate anti-proliferative activity on different cancer cell line but no significantly affect on normal PBMC was observed. Nuclear fragmentation was observed in a dose dependent fashion by DAPI staining on A549 cells treated with methanolic extract of &lt;em&gt;Spirogyra triplicata. &lt;/em&gt;We further looked into the chemo profiling of&lt;em&gt; Spirogyra triplicata &lt;/em&gt;by GCMS analysis. The result of GC-MS clearly indicates presence of nineteen major components and twenty-three minor components which have more or less bioactivity and would help in therapeutics in future. &lt;strong&gt;Conclusions:&lt;/strong&gt; In brief this study indicates for the first time that green algae&lt;em&gt; Spirogyra triplicata &lt;/em&gt;induces anti-proliferative activity specifically against A549 cell but not in normal PBMC. It can be concluded that&lt;em&gt; Spirogyra triplicata&lt;/em&gt; holds a great promise as a good repository of anti cancer compounds which may be used in future drug discovery.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">569</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ankita Mridha, Priya K Gopal, Santanu Paul* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Laboratory of Cell and Molecular Biology, Department of Botany, University Of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, INDIA.&lt;/p&gt;
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