<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Maria Carmen S. Tan</style></author><author><style face="normal" font="default" size="100%">Marissa G. Noel</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Glucosinolates, Degradation Products and Myrosinase Activity in Raphanus sativus Linn.</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">GC-FID</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Glucosinolates</style></keyword><keyword><style  face="normal" font="default" size="100%">Isothiocyanates</style></keyword><keyword><style  face="normal" font="default" size="100%">LC-ESI-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Myrosinase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">866-872</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;This research was conducted to assess the gluocosinolate (GSL), isothiocyanate (ITC) and myrosinase content in two cultivars of Raphanus sativus Linn. (white and red) roots. LC-ESIMS investigation was done on desulfated GSLs since this methodology has been previously established for efficient GSL analyses. The major GSLs: sinigrin &lt;strong&gt;(1)&lt;/strong&gt; 1,2-dihydroxy-2-phenylethyl glucosinolate &lt;strong&gt;(2)&lt;/strong&gt;, 4-hydroxyglucobrassicin &lt;strong&gt;(3)&lt;/strong&gt;, glucoraphasatin &lt;strong&gt;(4)&lt;/strong&gt; and 4-methoxyglucobrassicin &lt;strong&gt;(5)&lt;/strong&gt; were found in red radishes; whereas, only &lt;strong&gt;1&lt;/strong&gt; and &lt;strong&gt;4&lt;/strong&gt; were obtained in white radishes. Myrosinase was analyzed in the tubers due to its ability to catalyze and hydrolyze GSLs into ITCs. This β-thioglucosidase enzyme was found to be over 10 times more active in red tubers (2.05E-02 units) than in white radishes (1.55E-03 units) and the results were linked to the presence/absence of the outer covering of the tubers. Due to the promising medicinal properties of the aglucone derivative of compound 4, 4-methylthio-3-butenyl isothiocyanate &lt;strong&gt;(6)&lt;/strong&gt;, the ITC analog was monitored using gas chromatographic mass spectral analyses after myrosinase-mediated hydrolysis. From the results, it can be construed that the occurrence of GSLs&lt;strong&gt; 1-5&lt;/strong&gt; and the bioactive agent &lt;strong&gt;6&lt;/strong&gt; were inherent in the R. sativus cultivars evaluated.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">866</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">866</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Maria Carmen S. Tan*, Marissa G. Noel&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Chemistry Department, De La Salle University, 2401 Taft Avenue, Manila 1004, PHILIPPINES.&lt;/p&gt;
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