<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Anu Avudaiappan</style></author><author><style face="normal" font="default" size="100%">Krittika Chandran</style></author><author><style face="normal" font="default" size="100%">Sajna Keeyari Purayil</style></author><author><style face="normal" font="default" size="100%">Abiramy Krishnan</style></author><author><style face="normal" font="default" size="100%">Maimuna Gai</style></author><author><style face="normal" font="default" size="100%">Aishath Aala Rasheed</style></author><author><style face="normal" font="default" size="100%">Soh Lee May</style></author><author><style face="normal" font="default" size="100%">Nur Izzah Bt Tohar</style></author><author><style face="normal" font="default" size="100%">Jamal Moideen Muthu Mohamed</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Green-Synthesized Copper Nanoparticles from Azadirachta Indica for Antimicrobial Applications and Potential Visible-Light- Assisted Organic Load Reduction of POME</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Azadirachta indica</style></keyword><keyword><style  face="normal" font="default" size="100%">Copper nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">Disinfection</style></keyword><keyword><style  face="normal" font="default" size="100%">Green synthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Wastewater treatment</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2026</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2026</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">129-138</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Palm Oil Mill Effluent (POME) is a high-strength agro-industrial wastewater of serious environmental concern due to its high organic load and microbial contamination. &lt;strong&gt;Methods:&lt;/strong&gt; Copper nanoparticles (CuNPs) were synthesized using &lt;em&gt;Azadirachta indica&lt;/em&gt; (neem) leaf extract and copper sulfate at different extract-to-metal salt ratios (1:1 and 2:1). The formation of CuNPs was confirmed by color change, FESEM, and EDX analyses. POME samples were collected from two treatment stages (raw and acidification ponds), and bacteria were isolated and identified using morphological, Gram staining, and biochemical tests. The antimicrobial activity of CuNPs at two concentrations (0.008 g and 0.016 g) was evaluated against isolated bacterial strains (&lt;em&gt;Bacillus &lt;/em&gt;spp., Pseudomonas spp., and Stenotrophomonas spp.) using the agar well diffusion method. &lt;strong&gt;Results: &lt;/strong&gt;The synthesized CuNPs ranged from 11–40 nm in size, confirming the presence of elemental copper with bioorganic capping. Antibacterial activity increased with CuNP loading, with the maximum inhibition zone (50 mm) observed for &lt;em&gt;Bacillus &lt;/em&gt;spp. using CuNPs synthesized from a 0.1 M precursor solution and 0.016 g nanoparticle dose. At higher precursor concentrations (1.0 M), aggregation of nanoparticles occurred, leading to reduced antibacterial efficiency. Neem-CuNPs therefore demonstrated strong antimicrobial (disinfection) activity, while photocatalytic effects were observed as a secondary, light-assisted reduction of organic content rather than direct biodegradation. &lt;strong&gt;Conclusion:&lt;/strong&gt; The green-synthesized CuNPs offer an eco-friendly and effective antimicrobial strategy for managing POME-associated bacteria. These findings provide a basis for future work combining CuNPs with biological systems to enhance wastewater remediation.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">129</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Anu Avudaiappan&lt;sup&gt;1&lt;/sup&gt;, Krittika Chandran&lt;sup&gt;1&lt;/sup&gt;, Sajna Keeyari Purayil&lt;sup&gt;1&lt;/sup&gt;, Abiramy Krishnan&lt;sup&gt;1&lt;/sup&gt;, Maimuna Gai&lt;sup&gt;1&lt;/sup&gt;, Aishath Aala Rasheed&lt;sup&gt;1&lt;/sup&gt;, Soh Lee May&lt;sup&gt;1&lt;/sup&gt;, Nur Izzah Bt Tohar&lt;sup&gt;1&lt;/sup&gt;, Jamal Moideen Muthu Mohamed&lt;sup&gt;2*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy &amp;amp; BioMedical Sciences, MAHSA University, Bandar Saujana Putra, 42610 Jenjarom, Selangor, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Manipal University College Malaysia, Jalan Batu Hampar, Bukit Baru, 75150 Melaka, MALAYSIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mayowa Oladele Agunbiade</style></author><author><style face="normal" font="default" size="100%">Sabiu Saheed</style></author><author><style face="normal" font="default" size="100%">Esta Van Heerden</style></author><author><style face="normal" font="default" size="100%">Carolina Henritta Pohl</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vivo Toxicopathological Evaluation of a Purified Bioflocculant Produced by Arthrobacter humicola</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Actinomycetes</style></keyword><keyword><style  face="normal" font="default" size="100%">Arthrobacter humicola</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioflocculant</style></keyword><keyword><style  face="normal" font="default" size="100%">Hematological</style></keyword><keyword><style  face="normal" font="default" size="100%">Polysaccharides</style></keyword><keyword><style  face="normal" font="default" size="100%">Wastewater treatment</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">486-492</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; In spite of the commendable flocculating activities of microbial flocculants, a good number of them potentiate significant toxicity. This study evaluated the &lt;em&gt;in vivo&lt;/em&gt; toxicological implications of treatment with the Purified bioflocculant (PB) from &lt;em&gt;Arthrobacter humicola&lt;/em&gt; using OECD guidelines. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; In the acute toxicity assessment, a single oral administration of 2000 mg/kg body weight (b.w.) of PB was given to the Wistar rats and the animals were observed for 2 weeks. The daily dose toxicity testing was performed through daily oral treatment with graded doses (50, 200 and 500 mg/kg b.w.) of PB for 4 weeks. Clinical signs of toxicity, behavioral changes, hematological and biochemical parameters were thereafter evaluated. &lt;strong&gt;Results:&lt;/strong&gt; PB at 2000 mg/kg b.w. produced no treatment-mediated signs of toxicity, behavioral changes or mortality in the animals. Thus, its no-observed-adverse-effect level was estimated to be above 2000 mg/kg b.w. In the repeated dose toxicity testing, treatments with PB also revealed no significant differences in the feeding patterns, lipid profiles, hematological and clinical biochemistry parameters when compared with the control group. Although, at 500 mg/kg b.w. PB, a significant increase was observed in the serum activities of alkaline phosphatase, nonetheless, cage side observations recorded no treatment-induced signs of toxicity and macro-histopathological examinations of all the investigated organs also revealed no obvious morphological changes. &lt;strong&gt;Conclusion:&lt;/strong&gt; The overall results suggested that PB was well tolerated by the animals and is endowed with monosaccharides bearing functional groups of flocculation importance, thus, suggesting its potential application as a safe actinomycetes bioflocculant for water treatment.&amp;nbsp;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">486</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Mayowa Oladele Agunbiade&lt;sup&gt;1,2*&lt;/sup&gt;, Sabiu Saheed&lt;sup&gt;1,3&lt;/sup&gt;, Esta Van Heerden&lt;sup&gt;1,4&lt;/sup&gt;, Carolina Henritta Pohl&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, 9301, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biocatalysis and Technical Biology Research Group, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Applied Sciences, Durban University of Technology, SOUTH AFRICA 4iWater Pyt Limited, Walter Sisulu 5, Bloemfontein, SOUTH AFRICA.&lt;/p&gt;
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