<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Evul Winoto Lukito</style></author><author><style face="normal" font="default" size="100%">Dyah Iswantini</style></author><author><style face="normal" font="default" size="100%">Budhi Antariksa</style></author><author><style face="normal" font="default" size="100%">Mohamad Rafi</style></author><author><style face="normal" font="default" size="100%">Setyanto Tri Wahyudi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening and Identification of Metabolites from Sambiloto (Andrographis paniculata) Ethanol Extract for Pro-Inflammatory Cytokines Inhibitory through In Silico and In Vitro Approaches</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">LC-MS/MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Pro-inflammatory cytokines</style></keyword><keyword><style  face="normal" font="default" size="100%">Sambiloto</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">131-140</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Andrographis paniculata has long been a traditional medicinal plant in Indonesia. This study is intended to evaluate the anti-pro-inflammatory cytokines of 98% ethanol extract of A. paniculata by &lt;em&gt;in vitro&lt;/em&gt; and &lt;em&gt;in silico&lt;/em&gt; approaches. Inhibition of pro-inflammatory cytokines is also one of the therapies in treating COVID-19. &lt;strong&gt;Methods: &lt;/strong&gt;The molecular docking approach was utilized as a first screening to evaluate the potential for suppression of macrophage cell activation; an ADMET prediction test was performed to determine the pharmacological, pharmacokinetic, and toxicity as a therapeutic target. TNF-α, IL-1ß, and IL-6 levels were measured using an ELISA method to investigate anti-cytokine pro-inflammatory activity in LPS-induced RAW 264.7 macrophage cells. LC-MS/MS was used to identify additional metabolite compounds. &lt;strong&gt;Results:&lt;/strong&gt; Ethanol extract containing particular metabolites 14-Deoxyandrographoside and 14-Deoxy-17-hydroxyandrographolide inhibited TNF-α and IL-1β by 100% and IL-6 by 85.59%, respectively. While compared to the Dexamethasone molecule as a positive control, preliminary screening and ADMET prediction for the metabolite compound 14-Deoxyandrographoside exhibited relatively high binding stability to the CD14 receptor by -7.5 kcal/mol and was safe against various ADMET indications. &lt;strong&gt;Conclusions: &lt;/strong&gt;This study reveals that the compound 14-Deoxyandrographoside in pure ethanol extract is a potential anticytokine agent candidate for treating pro-inflammatory cytokines, including COVID-19 infection.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">131</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Evul Winoto Lukito&lt;sup&gt;1&lt;/sup&gt;, Dyah Iswantini&lt;sup&gt;1,2&lt;/sup&gt;*, Budhi Antariksa&lt;sup&gt;3&lt;/sup&gt;, Mohamad Rafi&lt;sup&gt;1,2&lt;/sup&gt;, Setyanto Tri Wahyudi&lt;sup&gt;2,4&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Tropical Biopharmaca Research Center, IPB University, Bogor 16128, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan Hospital, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Physics, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kusmardi Kusmardi</style></author><author><style face="normal" font="default" size="100%">Baiqi Nur Hairi</style></author><author><style face="normal" font="default" size="100%">Nadar Sukri Lubis</style></author><author><style face="normal" font="default" size="100%">Tri Wahyuni Lestari</style></author><author><style face="normal" font="default" size="100%">Putri Reno Intan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Effect of Sambiloto and Spirulina Combination on Mucin-1 Protein Expression in Medial Colon of Plasmodium berghei ANKA Infected Mice</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Medial colon</style></keyword><keyword><style  face="normal" font="default" size="100%">Muc-1</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmodium berghei Anka</style></keyword><keyword><style  face="normal" font="default" size="100%">Sambiloto</style></keyword><keyword><style  face="normal" font="default" size="100%">Spirulina</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1359-1365</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Malaria still be health problem in the world, especially in Eastern Indonesia. Malaria’s inflammation and metabolism defect can cause colonic damage, such as enhancement Muc-1 protein expression and goblet cells hyperplasia. Sambiloto and spirulina combination as antiinflammatory and antioxidative agent can prevent medial colon damage &lt;em&gt;Plasmodium berghei &lt;/em&gt;ANKA infected mice. The aim of the study to show the effect of sambiloto and spirulina combination on Muc-1 protein activity in medial colon &lt;em&gt;Plasmodium berghei&lt;/em&gt; ANKA infected mice. This study use preserve male Swiss Webser mice colonic tissue which has inoculated by&lt;em&gt; Plasmodium berghei &lt;/em&gt;ANKA, whose treatment group include positive control (dehyroartemisin piperaquine), negative control (carboxymethil cellulose), AP (sambiloto), AP+ES (sambiloto+spirulina extract), and AP+PS (sambiloto+spirulina powder) and terminated after 28 days of treatment. Colonic tissue was stained with immunohistochemistry and observed using light microscope (400x) in five different field and was analyzed with ImageJ® sowtware, and statisitcal analysis was done with SPSS 20.0. According to One Way ANOVA and Duncan posthoc test, only AP+PS(120,98 ±3,37), which significantly difference between negative control, AP, and AP+ES group. Meanwhile, between DHP, AP+PS group not significantly differenece. Sambiloto extract and spirulina powder combination can reduce Muc-1 protein expression in medial colon &lt;em&gt;Plasmodium berghei &lt;/em&gt;ANKA infected mice.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1359</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kusmardi Kusmardi&lt;sup&gt;1-4&lt;/sup&gt;,*, Baiqi Nur Hairi&lt;sup&gt;5&lt;/sup&gt;, Nadar Sukri Lubis&lt;sup&gt;6&lt;/sup&gt;, Tri Wahyuni Lestari&lt;sup&gt;7&lt;/sup&gt;, Putri Reno Intan&lt;sup&gt;8&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Human Cancer Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Parasitology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;National Institute of Health Research and Development, Ministry of Health of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kusmardi Kusmardi</style></author><author><style face="normal" font="default" size="100%">Rahyussalim</style></author><author><style face="normal" font="default" size="100%">Rizky Priambodo Wisnubaroto</style></author><author><style face="normal" font="default" size="100%">Dilla Firzani</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Combination of Spirulina Extract and Sambiloto Effect Histopathologically on Medial Colon from Plasmodium berghei Anka Infected Mice</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Malaria</style></keyword><keyword><style  face="normal" font="default" size="100%">Medial colon</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmodium berghei Anka</style></keyword><keyword><style  face="normal" font="default" size="100%">Sambiloto</style></keyword><keyword><style  face="normal" font="default" size="100%">Spirulina</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">564-569</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Malaria is one of the biggest burden in medical section in Indonesia, as the prevalence is still high and half of the provinces in Indonesia considered as endemic area.&lt;sup&gt;1-4&lt;/sup&gt; Moreover, the drug resistant case number has grown larger within years.&lt;sup&gt;3-7&lt;/sup&gt; Indonesian people are very close with traditional drug regiment that derived from plants, for example &lt;em&gt;Sambiloto&lt;/em&gt; and &lt;em&gt;Spirulina.&lt;/em&gt;&lt;sup&gt;7-9 &lt;/sup&gt;This research done in order to see the effect of &lt;em&gt;Sambiloto and Spirulina&lt;/em&gt; combination from histopathologic aspect in medial colon of &lt;em&gt;P. berghei &lt;/em&gt;infected mice. &lt;strong&gt;Method:&lt;/strong&gt; The data taken from experimental study using male Swiss Webster mice that has been infected with &lt;em&gt;Plasmodium berghei &lt;/em&gt;Anka. The four groups of mice were given different treatment. The first group treated with &lt;em&gt;Sambiloto &lt;/em&gt;only, the second one with &lt;em&gt;Sambiloto&lt;/em&gt; and Spirulina extract, the third group with &lt;em&gt;Sambiloto and &lt;/em&gt;Spirulina powder and the last one the control group with administration of DHP. &lt;strong&gt;Results:&lt;/strong&gt; The result shows that the group with extract and powder spirulina show a significant result in the inflammatory focus and angiogenesis. However, this research does not necessarily prove the correlation between &lt;em&gt;Sambiloto&lt;/em&gt;-Spirulina and their effect on the goblet cell and dysplasia grade on the infected mice, as the result for both category is insignificant. &lt;strong&gt;Conclusion: &lt;/strong&gt;The study showed that spirulina has positive effect on inflamatory focus and angiogenesis, but the goblet cells count and dysplasia grade result is not noteworthy, as it requires prolonged inflammation process in order to achieve the optimal result.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">564</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kusmardi Kusmardi&lt;sup&gt;1&lt;/sup&gt;, Rahyussalim&lt;sup&gt;2&lt;/sup&gt;, Rizky Priambodo Wisnubaroto&lt;sup&gt;2,*&lt;/sup&gt;, Dilla Firzani&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pathological Anatomy, Faculty of Medicine Indonesia, Universitas Indonesia, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Undergraduate student Faculty of Medicine, Universitas Indonesia, INDONESIA.&lt;/p&gt;
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