<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ravikumar Shivakumar</style></author><author><style face="normal" font="default" size="100%">Krishna Venkatarangaiah</style></author><author><style face="normal" font="default" size="100%">Sudhesh Shastri</style></author><author><style face="normal" font="default" size="100%">Ravishankara Burladinni Nagaraja</style></author><author><style face="normal" font="default" size="100%">Ajith Sheshagiri</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antibacterial Property and Molecular Docking Studies of Leaf Calli Phytochemicals of Bridelia scandens Wild.</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ADMET</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Bridelia scandens</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Gyrase</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">1221-1229</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Bridelia scandens&lt;/em&gt; Wild. (Euphorbiaceae) leaves are widely used to cure asthma, bronchitis pleurisy, exudation, sores in mouth and genital cancers. &lt;strong&gt;Objective:&lt;/strong&gt; To evaluate antibacterial activity of the leaf calli methanol extract (LCME). &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Mass production of leaf calli was established on MS medium supplemented with 0.5 mg/L BAP and 0.5 mg/L 2, 4-D. Methanol extract of the dried calli was subjected to HR-LCMS analysis, antibacterial screening of the extract was carried out against human pathogenic clinical isolates. Molecular docking study of HR-LCMS identified compounds was performed by docking with bacterial enzyme DNA gyrase.&lt;strong&gt; Results:&lt;/strong&gt; HR-LCMS analysis of LCME shows that the compounds azaperone bifonazole, fusidic acid, lasalocid and quinine as the major constituents. The antibacterial screening of LCME against clinical pathogens showed significant bactericidal activity against the strains Staphylococcus aureus (17.67&amp;plusmn;0.88 mm.d.), &lt;em&gt;Streptococcus pneumonia&lt;/em&gt; (13.67&amp;plusmn;0.33), &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; (16.33&amp;plusmn;0.67), &lt;em&gt;Salmonella typhi&lt;/em&gt; (17.67&amp;plusmn;0.33), and Vibrio cholera (15.33&amp;plusmn;0.33) as compared to the standard drug ciprofloxacin. The molecular docking of lasalocid against the bacterial enzyme DNA gyrase exhibited good binding affinity of -4.9 kcal/mol, good drug likeness (2.5589), 2 hydrogen bonds and hydrophobic interaction with 7 amino acid residues, so that lasalocid processes good inhibitor as compared to other 4 compounds. &lt;strong&gt;Conclusion:&lt;/strong&gt; LCME of &lt;em&gt;Bridelia scandens&lt;/em&gt; showed significant antibacterial activity against &lt;em&gt;Staphylococcus aureus&lt;/em&gt; and &lt;em&gt;Salmonella typhi&lt;/em&gt;. Lasalocid is the major phytocomponent of LCME which exhibited good inhibitory activity against bacterial enzyme DNA gyrase. This investigation supported traditional claim of LCME as potential antibacterial drug.&amp;nbsp;&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1221</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Ravikumar Shivakumar, Krishna Venkatarangaiah, Sudhesh Shastri, Ravishankara Burladinni Nagaraja, Ajith Sheshagiri &lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Department of PG Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta, Shivamogga, Karnataka, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Preetham Jinadatta</style></author><author><style face="normal" font="default" size="100%">Kiran Sundera Raja Rao</style></author><author><style face="normal" font="default" size="100%">Sharath Rajshekarappa</style></author><author><style face="normal" font="default" size="100%">Sujan Ganapathy Pasura Subbaiah</style></author><author><style face="normal" font="default" size="100%">Mruthunjaya Kenganora</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Antioxidant and Hepatoprotective Activity of Bridelia scandens (Roxb.)Willd</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bridelia scandens</style></keyword><keyword><style  face="normal" font="default" size="100%">BRL3A</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepatoprotective</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT</style></keyword><keyword><style  face="normal" font="default" size="100%">ORAC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/392</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">s117-s121</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;Natural products are emerging out as potent and alternative therapies for many diseases. Today herbs have become the part of mankind, because of its manifold ways in targeting diseased cells with minimal effects on normal cells and tissues. The present research investigated the &lt;em&gt;in vitro&lt;/em&gt; antioxidant activity and hepatoprotective of &lt;em&gt;B.scandens&lt;/em&gt; leaf. Preliminary phytochemical analysis exhibited the presence of most of the constituent in ethanol extract (BSE). Antioxidant capacity of various extracts of &lt;em&gt;B.scandens&lt;/em&gt; was examined. DPPH assay revealed that ethanol extract has a good antioxidant with IC&lt;sub&gt;50&lt;/sub&gt; value of 31.68&amp;mu;g/ml, whereas standard ascorbic acid with 8.78 &amp;mu;g/ml. BSE revealed dose dependent response with increase in concentration for reducing power assay. ORAC assay directly measured the scavenging capacity and BSE (2485 trolox eq/gm) was found to be potent than other extracts. &lt;em&gt;In vitro&lt;/em&gt; hepatoprotective activity was performed for BSE using MTT assay in BRL 3A cell line, which revealed nontoxic dose with CTC&lt;sub&gt;50&lt;/sub&gt; value more than 1000 &amp;mu;g/ml. At the dose 200 &amp;mu;g/ml, BSE and standard silymarin offered cell protection of 57% and 76 % respectively. Present study concludes that &lt;em&gt;B.scandens&lt;/em&gt; leaf extract possess antioxidant potential and protect the liver cells against CCl&lt;sub&gt;4&lt;/sub&gt; damage. However in vivo studies are being carried out to validate the traditional usage of &lt;em&gt;Bridelia scandens&lt;/em&gt;.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">s117</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Preetham Jinadatta&lt;sup&gt;1&lt;/sup&gt;*, Kiran Sundera Raja Rao&lt;sup&gt;1&lt;/sup&gt;, Sharath Rajshekarappa&lt;sup&gt;2&lt;/sup&gt;, Sujan Ganapathy Pasura Subbaiah&lt;sup&gt;3&lt;/sup&gt;, Mruthunjaya Kenganora&lt;sup&gt;4 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biotechnology, Dayananda Sagar College of Engineering, Kumaraswamy Layout, Bangalore-560078, Karnataka, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, M.S. Ramaiah Institute of Technology, MSRIT Post Bangalore 560054, Karnataka, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Research and Development Centre, Indusviva International Private Limited, No.7450, Near Navayuga Toll Gate Office, NH-4, Nelamangala, Bangalore &amp;ndash; 562123, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacognosy, JSS College of Pharmacy, JSS University, Mysuru-570015 Karnataka, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>