<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Balachandran Karpaga Raja Sundari</style></author><author><style face="normal" font="default" size="100%">Srivani Telapolu</style></author><author><style face="normal" font="default" size="100%">Bilikere S</style></author><author><style face="normal" font="default" size="100%">Dwarakanath</style></author><author><style face="normal" font="default" size="100%">Sadras. P. Thyagarajan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic and Antioxidant Effects in Various Tissue Extracts of Plumbago zeylanica: Implications for Anticancer Potential</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549 Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Alcoholic Root Extract</style></keyword><keyword><style  face="normal" font="default" size="100%">DPPH assay</style></keyword><keyword><style  face="normal" font="default" size="100%">HPTLC Quantification</style></keyword><keyword><style  face="normal" font="default" size="100%">Plumbagin</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcriptome Analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/pj-9-5/10.5530pj.2017.5.111/index.html</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">706-712</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Complex natural products are emerging as a potent alternate and complement in anticancer therapies due to its multiple mechanisms of action and minimal effects on nontarget cells/tissues. Some of the traditional herbal formulations contain &lt;em&gt;Plumbago zeylanica &lt;/em&gt;L, (commonly known as Chitraka or Chitramoolam) to treat various disorders for centuries in Africa and Asia. &lt;strong&gt;Methods:&lt;/strong&gt; The quantity of Plumbagin was detected in different tissues of &lt;em&gt;P. zeylanica&lt;/em&gt; through HPTLC analysis. We evaluated and compared the alcoholic tissue extracts of leaf, stem and root of &lt;em&gt;P. zeylanica&lt;/em&gt; with standard pure Plumbagin for its &lt;em&gt;in vitro&lt;/em&gt; cytotoxic effects (metabolic viability; MTT assay) in A549 Lung cancer cells and antioxidant potential (DPPH free radical scavenging and total phenol content). RNA sequencing and transcriptome analysis was performed in the most potent tissues of &lt;em&gt;P. zeylanica&lt;/em&gt; demonstrating efficient pharmacological activity. &lt;strong&gt;Results:&lt;/strong&gt; The root extract containing 1.3% of plumbagin exhibited strongest scavenging potential with 50% radical scavenging (IC&lt;sub&gt;50&amp;nbsp;&lt;/sub&gt;value of 3.99 &lt;em&gt;&amp;mu;&lt;/em&gt;g) in comparison with 35.85 &lt;em&gt;&amp;mu;&lt;/em&gt;g in stem (0.2% Plumbagin) and 18.06 &lt;em&gt;&amp;mu;&lt;/em&gt;g in leaf with 0.00007% Plumbagin. The &lt;em&gt;in vitro&lt;/em&gt; cytotoxicity in A549 cells also showed that the root extract was most potent with an IC&lt;sub&gt;50&amp;nbsp;&lt;/sub&gt;value of 164.5&amp;nbsp;&lt;em&gt;&amp;mu;&lt;/em&gt;g as compared to extracts from stem (IC&lt;sub&gt;50&lt;/sub&gt;= 379.5&amp;mu;g) and leaf (IC50= 274.9 &lt;em&gt;&amp;mu;&lt;/em&gt;g). &lt;strong&gt;Conclusion:&lt;/strong&gt; The strong antioxidant potential shown by the &lt;em&gt;P. zeylanica &lt;/em&gt;extract demonstrates its ability to protect the non-target (normal) cells against oxidative stress, while the cytotoxic effects suggest that it could inhibit the growth of cancerous cells. Further, transcriptome analysis predicted the expression of potent gene transcripts coding for quinone biosynthesis in the root tissues of &lt;em&gt;P. zeylanica.&lt;/em&gt;&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">706</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Balachandran Karpaga Raja Sundari&lt;sup&gt;1*&lt;/sup&gt;, Srivani Telapolu&lt;sup&gt;2&lt;/sup&gt;, Bilikere S. Dwarakanath&lt;sup&gt;3&lt;/sup&gt;, Sadras. P. Thyagarajan&lt;sup&gt;4* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Ph.D. DST-National Post-Doctoral Fellow, Herbal and Indian Medicine Research Laboratory, Central Research Facility, Sri Ramachandra University, Porur, Chennai-600116, Tamil Nadu, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Ph.D Scholar, Herbal and Indian Medicine Research Laboratory, Central Research Facility, Sri Ramachandra University, Chennai-600116, Tamil INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Ph.D., Professor (Research), Central Research Facility, Sri Ramachandra University, Chennai-600 116, Tamil Nadu. INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Ph.D., M.D., D.Sc. Professor of Eminence &amp;amp; Dean (Research), Central Research Facility, Sri Ramachandra University, Chennai-600116, Tamil Nadu, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mohammadi M</style></author><author><style face="normal" font="default" size="100%">Majd A</style></author><author><style face="normal" font="default" size="100%">Nejadsattari T</style></author><author><style face="normal" font="default" size="100%">Hashemi M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antioxidant and Anticancer Activities of Ocimum basilicum L. cv. Dark Opal (Lamiaceae)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">3-(4</style></keyword><keyword><style  face="normal" font="default" size="100%">4 diphenyltetrazolium bromide assay</style></keyword><keyword><style  face="normal" font="default" size="100%">5-dimethylthiazol-2yl)-2</style></keyword><keyword><style  face="normal" font="default" size="100%">A549 Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Digera muricata</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cell line.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">3rd Sept, 2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">S</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Plants used in folk and traditional medicines have been accepted as therapeutic drug development in modern medicine.Although manystudies have been focused on Lamiaceae family,few studies on medical effects of purple basil have been performed until now. &lt;strong&gt;Objective:&lt;/strong&gt; Ocimum basilicum cv. dark opal was chosen for this study as it has been used in Persian traditional medicine and many Iranian dishes. It was considered important to determine the cytotoxicity effect andthe reductive capacity of the purple basil oils and extracts, as this may indicate their potential as antioxidants. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;The reducing power activity of both essential oils of the leaves (before flowering) and the seeds and also methanolic extracts of roots and aerial parts (stem-leaf) (collected prior flowering), and flowers were determined by utilizing of FRAP. Also the MTT assay has been used in order to consider in vitro cytotoxicity of essential oils and extracts on cancerous cell line (MCF-7). Moreover, the extracts were analyzed by HPLC to comprise the rate of some phenolic compounds.&lt;strong&gt; Results: &lt;/strong&gt;The purple basil extracts have more powerful antioxidant activity than the essential oils. MCF-7 revealed to have a meaningful cell death when compared with controls, and the oils were found to be more effective than the extracts. Also the phytochemical analysis of the extracts has led to the identification of 3 phenolic compounds (Rosmarinic acid, p-Coumaric acid and Ferulic acid). &lt;strong&gt;Conclusion:&lt;/strong&gt; Our study tends to validate the traditional use of this medicinal herb as complementary and alternative medicine.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key Words:&lt;/strong&gt; purple basil, antioxidant, MCF-7, HPLC.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">32</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Shazia Usmani&lt;sup&gt;*1&lt;/sup&gt;, Arshad Hussain&lt;sup&gt;1&lt;/sup&gt;, A. H. A. Farooqui&lt;sup&gt;2&lt;/sup&gt;, Mohammed Arshad&lt;sup&gt;3&lt;/sup&gt;, Sahabjada Siddiqui&lt;sup&gt;3&lt;/sup&gt;, Mohammed Ahmad&lt;sup&gt;1&lt;/sup&gt;, Shadma Wahab&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India, &lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, Faculty of Biosciences, Integral University, Lucknow, Uttar Pradesh, India, &lt;sup&gt;3&lt;/sup&gt;Department of Zoology, Faculty of Life Sciences, Lucknow University, Lucknow, Uttar Pradesh, India&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shazia Usmani</style></author><author><style face="normal" font="default" size="100%">Arshad Hussain</style></author><author><style face="normal" font="default" size="100%">A.H.A Farooqui</style></author><author><style face="normal" font="default" size="100%">Mohd.Arshad</style></author><author><style face="normal" font="default" size="100%">Sahabjada Siddiqui</style></author><author><style face="normal" font="default" size="100%">Mohd.Ahmad</style></author><author><style face="normal" font="default" size="100%">Shadma Wahab</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">3-(4</style></keyword><keyword><style  face="normal" font="default" size="100%">4 diphenyltetrazolium bromide assay</style></keyword><keyword><style  face="normal" font="default" size="100%">5-dimethylthiazol-2yl)-2</style></keyword><keyword><style  face="normal" font="default" size="100%">A549 Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Digera muricata</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cell line</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">3rd Sept, 2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">32-38</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;em&gt;Digera muricata&lt;/em&gt; (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A&lt;sub&gt;549&lt;/sub&gt; cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of &lt;em&gt;Digera muricata&lt;/em&gt; in a dose dependent manner ranging from 25&amp;mu;g/ml to 250&amp;mu;g/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of &lt;em&gt;Digera muricata&lt;/em&gt; can be good source of cytotoxic compounds.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A&lt;sub&gt;549&lt;/sub&gt; cell line, cytotoxic,
Digera muricata, HeLa cell line.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">32</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Shazia Usmani&lt;sup&gt;a*&lt;/sup&gt;&lt;sub&gt;, &lt;/sub&gt;Arshad Hussain&lt;sup&gt;a&lt;/sup&gt;, A.H.A Farooqui&lt;sup&gt;b&lt;/sup&gt;, Mohd.Arshad&lt;sup&gt;c&lt;/sup&gt;, Sahabjada Siddiqui&lt;sup&gt;c&lt;/sup&gt;, Mohd.Ahmad&lt;sup&gt;a&lt;/sup&gt;, Shadma Wahab&lt;sup&gt;a&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;sup&gt;a&lt;/sup&gt;Faculty of Pharmacy, Integral University, Kursi Road, Lucknow-226026, INDIA.&lt;/p&gt;&lt;p&gt;&lt;sup&gt;b&lt;/sup&gt;Faculty of Biosciences, Integral University, Kursi Road, Lucknow-226026, INDIA.&lt;/p&gt;&lt;p&gt;&lt;sup&gt;c&lt;/sup&gt;Department of Zoology, Faculty of Life Sciences, Lucknow University, Lucknow, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>