<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Manish Kumar</style></author><author><style face="normal" font="default" size="100%">Ankita Misra</style></author><author><style face="normal" font="default" size="100%">Akanksha Srivastava</style></author><author><style face="normal" font="default" size="100%">Pushpendra Kumar Shukla</style></author><author><style face="normal" font="default" size="100%">L M Tewari</style></author><author><style face="normal" font="default" size="100%">Sharad Srivastava</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative Pharmacognostical and Pharmacological Evaluation of Costus speciosus (Koen) J.E. Sm. Germplasm Collected from Eastern Ghats of India</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-diabetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-inflammatory</style></keyword><keyword><style  face="normal" font="default" size="100%">Costus speciosus</style></keyword><keyword><style  face="normal" font="default" size="100%">Diosgenin</style></keyword><keyword><style  face="normal" font="default" size="100%">HPTLC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">150-156</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Costus speciosus &lt;/em&gt;is an erect perennial herb belonging to family Costaceae, an important medicinal plant widely used in several indigenous medicinal formulations. &lt;strong&gt;Objective: &lt;/strong&gt;A comparative evaluation of Pharmacognostical and Pharmacological potential of &lt;em&gt;Costus speciosus &lt;/em&gt;for the validation of traditional claims and quality parameters for industry. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Pharmacognostical studies were performed as per Ayurvedic Pharmacopeia of India and quantification of diosgenin was done through HPTLC. &lt;em&gt;In vitro&lt;/em&gt; antidiabetic activity was evaluated by α-amylase inhibition assay based on starch iodine method and &lt;em&gt;in vitro &lt;/em&gt;anti-inflammatory were done by using inhibition of protein denaturation assay. &lt;strong&gt;Results: &lt;/strong&gt;The pharmacognostical standards were also laid down for each sample. Morpho-anatomical characters had no distinct variation in all the collected samples of Eastern Ghats. The quantification of diosgenin (without hydrolysis of samples) in the collected germplasm varies significantly from 0.002 to 0.076 % on dry weight basis. The maximum content was recorded in NBCS-06 from Patiya, Bhubaneswar and was identified as distinct chemotype with high metabolite content. IC&lt;sub&gt;50&lt;/sub&gt; value of&lt;em&gt; Costus speciosus &lt;/em&gt;extract in starch-iodine assay was found to be maximum in NBCS- 6 (87.54 μg/ml) and inhibition of protein denaturation assay was found to be maximum in NBCS- 11 (73.91 μg/ml), respectively. &lt;strong&gt;Conclusion: &lt;/strong&gt;The study suggests that the &lt;em&gt;Costus speciosus&lt;/em&gt; germplasm possess potential anti-inflammatory and anti-diabetic activity and comparative pharmacognostical parameters will be useful in collection of location specific potential samples for industrial usage along with quality control of raw materials.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">150</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Manish Kumar&lt;sup&gt;1,2&lt;/sup&gt;, Ankita Misra&lt;sup&gt;1&lt;/sup&gt;, Akanksha Srivastava&lt;sup&gt;1&lt;/sup&gt;, Pushpendra Kumar Shukla&lt;sup&gt;1&lt;/sup&gt;, L. M. Tewari&lt;sup&gt;2&lt;/sup&gt;, Sharad Srivastava&lt;sup&gt;1,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow (U.P.) 226001, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Botany, D.S.B. Campus, Kumaun University, Nainital-263002, Uttarakhand, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pushpendra Kumar Shukla</style></author><author><style face="normal" font="default" size="100%">Ankita Misra</style></author><author><style face="normal" font="default" size="100%">Sharad Srivastava</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative Pharmacognostical and Pharmacological Evaluation of two Achyranthes species</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacog Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Achyranthes</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">HPTLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Linoleic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Oleanolic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">α- amylase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January-2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/484</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">309-314</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Achyranthes&lt;/em&gt; is a well-known herb used in folk lore and traditional systems of medicine for its therapeutic value. The two species &lt;em&gt;Achyranthes aspera&lt;/em&gt; and &lt;em&gt;Achyranthes bidentata&lt;/em&gt; are used interchangeably by people and by herbal industries due to their resemblance in appearance. Therefore, the present study was undertaken to evaluate the comparative pharmacognostic and pharmacological properties of both species. &lt;strong&gt;Methods:&lt;/strong&gt; Pharmacognostic characters were evaluated as per the guidelines of Ayurvedic Pharmacopoeia of India. A quantitative HPTLC method was developed for quantification of linoleic acid and oleanolic acid using toluene: ethyl acetate: formic acid (6: 4: 0.5 v/v/v) as a mobile phase. Quantification was performed using linear regression analysis by plotting the peak area vs concentration curve with 2000-5000 ng/band (R&lt;sup&gt;2&lt;/sup&gt; = 0.998) for oleanolic acid and 2000-5000 ng/band (R&lt;sup&gt;2&lt;/sup&gt; = 0.994) for linoleic acid. The developed method was validated in terms of accuracy, recovery and inter and intraday study as per ICH guidelines. Antioxidant activity of methanolic extracts was estimated by five different models viz. DPPH free radical scavenging assay, total anti-oxidant capacity, reducing power assay, total flavonoid and phenol content. Anti-diabetic activity was analyzed by &amp;alpha;-amylase inhibition assay using 3, 5 di nitro salicylic acid and iodine starch model. &lt;strong&gt;Results:&lt;/strong&gt; The limit of detection (LOD) and limit of quantification (LOQ) of oleanolic acid and linoleic acid were determined, respectively, as 0.426, 1.29 and 0.427, 1.29 &amp;mu;g mL&amp;minus;1. Inhibition of free radicals increases with concentration and IC&lt;sub&gt;50&lt;/sub&gt; of &lt;em&gt;A. aspera and A. bidendata&lt;/em&gt; was obtained at 1.35 &amp;plusmn; 0.173 mg/ml and 1.28 &amp;plusmn; 0.169 mg/ml respectively. In &lt;em&gt;in vitro&lt;/em&gt; antidiabetic activity, IC&lt;sub&gt;50&lt;/sub&gt; value shows that &lt;em&gt;A. bidentata&lt;/em&gt; exhibit better activity than &lt;em&gt;A. aspera.&lt;/em&gt; &lt;strong&gt;Conclusion:&lt;/strong&gt; The present study generates data for the proper establishment of quality control standards of the crude drug.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">309</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Pushpendra Kumar Shukla, Ankita Misra, Sharad Srivastava&lt;/strong&gt;&lt;sup&gt;&lt;strong&gt;*&lt;/strong&gt; &lt;/sup&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow-226001, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Akanksha Srivastava</style></author><author><style face="normal" font="default" size="100%">Kuldeep Awasthi</style></author><author><style face="normal" font="default" size="100%">Bhanu Kumar</style></author><author><style face="normal" font="default" size="100%">Ankita Misra</style></author><author><style face="normal" font="default" size="100%">Sharad Srivastava</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pharmacognostic and Pharmacological Evaluation of Hyssopus officinalis L. (Lamiaceae) Collected from Kashmir Himalayas, India</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">DPPH</style></keyword><keyword><style  face="normal" font="default" size="100%">HPTLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyssopus officinalis</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenolic acids</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/652</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">690-693</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Hyssopus officinalis&lt;/em&gt; L. is a well-known herb for its culinary and medicinal significance. The purpose of this study was to perform the pharmacognostic evaluation. &lt;strong&gt;Methods:&lt;/strong&gt; Physicochemical and phytochemical analysis, HPTLC quantification and &lt;em&gt;in vitro&lt;/em&gt; antioxidant and antidiabetic activity were done. Results: Preliminary screening revealed the presence of phytomolecules such as alkaloid (0.99%), tannin (1.75%), sugar (1.96%) and starch (0.68%). Total phenolic and flavonoid content were found to be 2.32% and 1.16% respectively. HPTLC quantification data showed that the content of ferulic acid (0.034%) was higher than caffeic acid (0.0064%) on dry weight basis The IC&lt;sub&gt;50&lt;/sub&gt; value for the &lt;em&gt;in vitro&lt;/em&gt; DPPH radical scavenging assay was 0.50 &amp;mu;g/ml and &lt;em&gt;in vitro&lt;/em&gt; anti diabetic assay displayed IC50 value of 0.8366 mg/ml. &lt;strong&gt;Conclusion:&lt;/strong&gt; The study suggests presence of considerable amount of phenolic acids and antioxidant activity in the plant which supports its use in the traditional systems of medicine.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">690</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Akanksha Srivastava, Kuldeep Awasthi, Bhanu Kumar, Ankita Misra, Sharad Srivastava&lt;/strong&gt;&lt;sup&gt;&lt;strong&gt;*&lt;/strong&gt; &lt;/sup&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Pharmacognosy and Ethnopharmacology Division CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ankita Misra</style></author><author><style face="normal" font="default" size="100%">Akanksha Srivastava</style></author><author><style face="normal" font="default" size="100%">Mohammad Khalid</style></author><author><style face="normal" font="default" size="100%">Poonam Kushwaha</style></author><author><style face="normal" font="default" size="100%">Sharad Srivastava</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Evaluation of Anti Arthritic Potential of Gloriosa superba (L.) Elite Germplasm Collected from Eastern Himalayas, India</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti arthritic</style></keyword><keyword><style  face="normal" font="default" size="100%">Colchicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Elite chemotype</style></keyword><keyword><style  face="normal" font="default" size="100%">G. superba</style></keyword><keyword><style  face="normal" font="default" size="100%">HPTLC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/387</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">s87-s92</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Gloriosa superba&lt;/em&gt; (L.) is a traditionally known medicinal plant for its potential antigout property. The species is rich source of colchicine alkaloid and is commercially exploit in the international market for the same. &lt;strong&gt;Method:&lt;/strong&gt; In the present study, elite chemotype of &lt;em&gt;G. superba&lt;/em&gt; was identified from natural population in Eastern Himalayas based on their colchicine content through HPTLC calibrated method. The selected elite chemotypes were further evaluated for &lt;em&gt;in vitro&lt;/em&gt; anti-arthritic potential via inhibition of protein denaturation along with hydroxyl radical scavenging potential. &lt;strong&gt;Result:&lt;/strong&gt; The HPTLC quantification data reveals that the content of colchicine varies from 0.044 to 0.184% having maximum content in NBG-128 from Jorhat, Assam. The results of bioassay reflect a potentiating anti-arthritic and hydroxyl radical scavenging with statistically insignificant difference within the elite germplasms. &lt;strong&gt;Conclusion:&lt;/strong&gt; The presence of bioactive polyphenolics with significant hydroxyl radical scavenging will further suggest that inhibition of inflammatory mediator cells by extract is superimposed action of colchicine and other chemical inhibitors like polyphenolics. The study will aid in site specific exploration of high metabolite yielding chemotype(s) with validated pharmacological action for commercial cultivation to meet out the industrial demand of colchicine and herbal product development.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">s87</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Ankita Misra&lt;sup&gt;1,2,&lt;/sup&gt; Akanksha Srivastava&lt;sup&gt;1&lt;/sup&gt;, Mohammad Khalid&lt;sup&gt;2&lt;/sup&gt;, Poonam Kushwaha&lt;sup&gt;2&lt;/sup&gt;, Sharad Srivastava&lt;sup&gt;1&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh 226001, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226001, INDIA.&amp;nbsp;&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ankita Misra</style></author><author><style face="normal" font="default" size="100%">Pushpendra Kumar Shukla</style></author><author><style face="normal" font="default" size="100%">Bhanu Kumar</style></author><author><style face="normal" font="default" size="100%">Abhishek Niranjan</style></author><author><style face="normal" font="default" size="100%">AKS Rawat</style></author><author><style face="normal" font="default" size="100%">Sharad Srivastava</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simultaneous-HPLC Quantification of Phenolic Acids in Traditionally used Ayurvedic Herb Diplocyclos palmatus (L.) Jeffry</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti diabetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti oxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Diplocyclos palmatus</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenolic acid</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/PJ-9-4/10.5530pj.2017.4.78</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">483-487</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;The present study deals with the simultaneous HPLC-quantification of phenolic acid(s) in the aerial parts of &lt;em&gt;Diplocyclos palmatus &lt;/em&gt;(Cucurbitaceae) and evaluation of their bioactivity potential through &lt;em&gt;in vitro &lt;/em&gt;antioxidant assay&amp;rsquo;s. &lt;strong&gt;Method:&lt;/strong&gt; The HPLC elution was done using C&lt;sub&gt;18&lt;/sub&gt; column using gradient (binary phases) solvent system at a flow rate of 0.6 ml/min. Total phenolic and, flavonoid contents were determined and the antioxidant potential was estimated by four assay&amp;rsquo;s viz. DPPH radical scavenging assay, ferric reducing power assay, total antioxidant capacity and 2-deoxy ribose assay. &lt;strong&gt;Results:&lt;/strong&gt; The species is rich in three phenolic acids, among which gallic acid (1708 ug/g) is in maximum concentration followed by caeffic acid (437 ug/g) and protocateuchic acid (337.7 ug/g). Total phenolic content was higher (10.5 mg/g) than flavonoid content (3.78 mg/g) and TAC was found at 0.137 mg/g ASE (ascorbic acid equivalent). IC&lt;sub&gt;50&lt;/sub&gt; of &lt;em&gt;D. palmatus &lt;/em&gt;extract for scavenging of hydroxyl radical by 2-deoxy ribose and DPPH was at concentration of 125.61 &amp;plusmn; 0.834 (&amp;mu;g/ml) and 353.71 &amp;plusmn; 0.663 (&amp;mu;g/ml) respectively. &lt;em&gt;In vitro &lt;/em&gt;antidiabetiv potential, via inhibition of alpha amylase enzyme through starch iodine and 3,5- DNS assay reveals the IC&lt;sub&gt;50&lt;/sub&gt; of extract at 146.31 &amp;plusmn; 0.415 ug/ml and 286.23 &amp;plusmn; 0.671 ug/ ml respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt;&amp;nbsp;The species (aerial part) was rich in phenolic acid with potential bioactivity, identified leads will be useful&amp;nbsp;in further chemical characterization and pharmacological validation.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><section><style face="normal" font="default" size="100%">483</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Ankita Misra, Pushpendra Kumar Shukla, Bhanu Kumar, Abhishek Niranjan, AKS Rawat and Sharad Srivastava&lt;sup&gt;* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;em&gt;Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute Lucknow (U.P.) 226001, INDIA.&lt;/em&gt;&lt;/p&gt;</style></auth-address></record></records></xml>