<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rajendra Prasad Gujjeti</style></author><author><style face="normal" font="default" size="100%">Estari Mamidala</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-HIV Activity of Phytosterol Isolated from Aerva lanata Roots</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Aerva lanata</style></keyword><keyword><style  face="normal" font="default" size="100%">Characterization</style></keyword><keyword><style  face="normal" font="default" size="100%">HIV-1</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytosterols</style></keyword><keyword><style  face="normal" font="default" size="100%">Reverse transcriptase</style></keyword><keyword><style  face="normal" font="default" size="100%">Structural elucidation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">112-116</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; AIDS in humans, which is caused by the HIV remains among the leading causes of death worldwide. &lt;strong&gt;Objective:&lt;/strong&gt; The study was undertaken to investigate the HIV-RT inhibitory activity of Phytotesrols extracted from roots of &lt;em&gt;Aerva lanata&lt;/em&gt;. &lt;strong&gt;Methodology:&lt;/strong&gt; Sequential maceration method was performed for preparation of extracts using hexane, chloroform, ethyl acetate, acetone and methanol solvents. RetroSys HIV-1 RT (Innovagen, Sweden) kit was used to determine the HIV-RT inhibitory activity of all solvents extracts. &lt;strong&gt;Results &amp;amp; Discussion:&lt;/strong&gt;&amp;nbsp;All extracts showed significant inhibitory activity. Chloroform extracts shows highest inhibition of recombinant HIV-RT (89.0%) at 2 and mg/ml concentration. Phytosterols isolated from chloroform extracts. These results were remarkable and can be used to develop a new drug for HIV treatment or other infectious diseases caused by the pathogenic micro-organisms, for which plant sterols may play a distinctive role. &lt;strong&gt;Conclusion:&lt;/strong&gt; Further work is in progress to evaluate the advanced spectroscopic studies are required for the structural elucidation, identification and characterization of the active compound from this plant.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">112</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Rajendra Prasad Gujjeti and Estari Mamidala* &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Department of Zoology, Infectious Diseases and Metabolic Disorders Research Lab, Kakatiya University, Warangal-506009, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Swapna Gurrapu</style></author><author><style face="normal" font="default" size="100%">Estari Mamidala</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Antibacterial Activity of Alkaloids Isolated from Leaves of Eclipta alba Against Human Pathogenic Bacteria</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agarwell diffusion</style></keyword><keyword><style  face="normal" font="default" size="100%">Alkaloid</style></keyword><keyword><style  face="normal" font="default" size="100%">Antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Broth micro-dilution assay.</style></keyword><keyword><style  face="normal" font="default" size="100%">Eclipta alba</style></keyword><keyword><style  face="normal" font="default" size="100%">Human pathogenic bacteria</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/PJ-9-4/10.5530pj.2017.4.91</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">573-577</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 &amp;mu;g/ml against all the bacteria. In this study, the &lt;em&gt;S. aureus &lt;/em&gt;and &lt;em&gt;E. coli&lt;/em&gt; are the more susceptible than the other selected human pathogenic bacteria. Based on the observations, &lt;em&gt;E. alba&lt;/em&gt; appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections. &lt;strong&gt;Context:&lt;/strong&gt; Different parts of Eclipta alba crude extracts are used traditionally for the treatment of several diseases of liver, skin and stomach. &lt;strong&gt;Aims:&lt;/strong&gt; To study the &lt;em&gt;in vitro&lt;/em&gt; antimicrobial activity of alkaloids isolated from &lt;em&gt;E. alba&lt;/em&gt; against human pathogenic bacteria. &lt;strong&gt;Settings and Design:&lt;/strong&gt; The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. &lt;strong&gt;Methods and Material:&lt;/strong&gt; The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. Agar-well diffusion and Broth micro-dilution methods were selected to assess the antimicrobial activity. &lt;strong&gt;Statistical analysis used:&lt;/strong&gt; One-way analysis of variance (ANOVA) was used to determine statistical significance. &lt;strong&gt;Results:&lt;/strong&gt; In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 &amp;mu;g/ml against all the bacteria. In this study, the &lt;em&gt;S. aureus&lt;/em&gt; and &lt;em&gt;E. coli&lt;/em&gt; are the more susceptible than the other selected human pathogenic bacteria. &lt;strong&gt;Conclusion:&lt;/strong&gt; Based on the observations, &lt;em&gt;E. alba&lt;/em&gt; appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">573</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Swapna Gurrapu and Estari Mamidala&lt;sup&gt;* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;em&gt;Infectious Diseases Research Lab, Department of Zoology, Kakatiya University, Warangal- 506009, telangana State, INDIA.&lt;/em&gt;&lt;/p&gt;</style></auth-address></record></records></xml>