<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">L S Ngidi</style></author><author><style face="normal" font="default" size="100%">C I Nxumalo</style></author><author><style face="normal" font="default" size="100%">J S Shandu</style></author><author><style face="normal" font="default" size="100%">T S Maliehe</style></author><author><style face="normal" font="default" size="100%">K Rene</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antioxidant, Anti-quorum Sensing and Cytotoxic Properties of the Endophytic Pseudomonas aeruginosa CP043328.1 's Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-quorum sensing activity</style></keyword><keyword><style  face="normal" font="default" size="100%">antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemical composition</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Pseudomonas aeruginosa CP043328.1</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">332-340</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Novel bioactive compounds are in high demand to combat challenges of microbial resistance. In recent years, secondary metabolites from endophytic bacteria have drawn attention from researchers due to their novel structures and significant biological activities. &lt;strong&gt;Objective:&lt;/strong&gt; This study aimed at extracting secondary metabolites from endophytic &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; CP043328.1 from &lt;em&gt;Anredera cordifolia&lt;/em&gt; leaves for their biological activities. &lt;strong&gt;Methods:&lt;/strong&gt; The anti-SMASH was used to predict the biosynthetic gene clusters of &lt;em&gt;P. aeruginosa &lt;/em&gt;CP043328.1. The bacteria was resuscitated on Nutrient agar. Ethyl acetate was used to extract the secondary metabolites. Chemical composition of the secondary metabolites was evaluated using gas chromatography-mass spectrometry (GC-MS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH), and 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods were used to analyze the scavenging activity. Anti-quorum sensing activity was investigated using &lt;em&gt;Chromobacterium violacuem &lt;/em&gt;ATCC 12472 as the biological indicator. Cytotoxicity assay was performed using a tetrozolium-based columetric (MTT) assay. &lt;strong&gt;Results: &lt;/strong&gt;The bacterium has 12 gene clusters that encode for secondary metabolites. The extract revealed 15 volatile constituents with diisooctyl phthalate (50.51%) and [1, 2, 4] oxadiazole, 5-benzyl-3 (10.44%) being the major compounds. The extract revealed scavenging capabilities with IC&lt;sub&gt;50&lt;/sub&gt; of 0.625 mg/ml for DPPH and 0.15 mg/ml for ABTS. It displayed anti-quorum sensing activity with 88% violacein inhibition at 3.31 mg/ml. According to MTT assay, the extract was found to be safe for use up to 2000 μg/mL. &lt;strong&gt;Conclusion:&lt;em&gt; &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;P. aeruginosa &lt;/em&gt;CP043328.1 shows a potential use as a source of pharmacologically important metabolites.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">332</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;L. S. Ngidi&lt;sup&gt;1,&lt;/sup&gt;*, C. I. Nxumalo&lt;sup&gt;1&lt;/sup&gt;, J. S. Shandu&lt;sup&gt;1&lt;/sup&gt;, T.S. Maliehe&lt;sup&gt;1&lt;/sup&gt;, K. Rene&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Discipline of Medical Biochemistry, College of Health Sciences, University of KwaZulu- Natal, Private Bag X 54001, Durban 4000, SOUTH AFRICA.&lt;/p&gt;
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