<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hesham I Elaskary</style></author><author><style face="normal" font="default" size="100%">Omar M Sabry</style></author><author><style face="normal" font="default" size="100%">Asmaa M Khalil</style></author><author><style face="normal" font="default" size="100%">Soheir M El Zalabani</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">UPLC-PDA-ESI-MS/MS Profiling of Clerodendrum inerme and Clerodendrum splendens and Significant Activity Against Mycobacterium tuberculosis</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Clerodendrum</style></keyword><keyword><style  face="normal" font="default" size="100%">Diterpenoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Iridoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenyl-propanoids</style></keyword><keyword><style  face="normal" font="default" size="100%">UPLC profiling</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1518-1524</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Antibiotic resistance is a major problem that is spreading and increasing while the development of new antibiotics is ceasing. As a result, some bacterial infections that were easily treated previously became untreatable. The antibacterial activity of &lt;em&gt;Clerodendrum inerme&lt;/em&gt; and &lt;em&gt;Clerodendrum splendens &lt;/em&gt;leaves were investigated against Mycobacterium tuberculosis, the widely known multi-drug resistant bacterium. UPLC-PDA-ESI-MS/MS is characterized by high sensitivity, resolution and speed for identification of plant metabolites even the minor ones. The chemical constituents of the leaves of &lt;em&gt;C. inerme &lt;/em&gt;and&lt;em&gt; C. splendens&lt;/em&gt; were investigated by UPLC-PDA-ESI-MS/MS metabolic analysis. &lt;strong&gt;Methods: &lt;/strong&gt;The antibacterial activity of the ethanol extracts of the leaves of the two species under investigation was evaluated against the multi-ethanol drug resistant bacterium &lt;strong&gt;Mycobacterium tuberculosis&lt;/strong&gt; using MABA assay. The methanol extracts of the leaves of &lt;em&gt;C. inerme&lt;/em&gt; and &lt;em&gt;C. splendens&lt;/em&gt; were subjected to comparative UPLC-PDA-ESI-MS/MS analysis. &lt;strong&gt;Results: &lt;/strong&gt;The ethanol extract of C .inerme leaves showed significant antibacterial activity against &lt;strong&gt;Mycobacterium tuberculosis&lt;/strong&gt;, while that of &lt;em&gt;C. splendens&lt;/em&gt; showed moderate activity. The UPLC-PDA-ESI-MS/MS analysis revealed a total of 36 metabolites detected and tentatively identified in the two species under investigation, among them 28 chromatographic peaks were assigned in&lt;em&gt; C. inerme&lt;/em&gt; while only 14 were assigned in these &lt;em&gt;C. splendens.&lt;/em&gt; The main classes of secondary metabolites detected were Phenylpropanoid and, iridoid glycosides, flavonoids, diterpenoids, phenolic acid and fatty acid derivatives. &lt;strong&gt;Conclusion: &lt;/strong&gt;The results of the antibacterial activity and UPLC-PDA-ESI-MS/ MS analysis showed stronger activity and higher number of metabolites for &lt;em&gt;C. inerme&lt;/em&gt; as compared to &lt;em&gt;C. splendens.&lt;/em&gt;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1518</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Hesham I. Elaskary, Omar M. Sabry*, Asmaa M. Khalil, Soheir M. El Zalabani &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, EGYPT.&lt;/p&gt;
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