<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ratika Rahmasari</style></author><author><style face="normal" font="default" size="100%">Takahiro Haruyama</style></author><author><style face="normal" font="default" size="100%">Muhareva Raekiansyah</style></author><author><style face="normal" font="default" size="100%">Farhana Mossadeque</style></author><author><style face="normal" font="default" size="100%">Marina Ika Irianti</style></author><author><style face="normal" font="default" size="100%">Ayun Erwina Arifianti</style></author><author><style face="normal" font="default" size="100%">Nobuyuki Kobayashi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Establishment of Simple Cell-based Screening Assay and the Identification of Potent Antiviral Activity of a Plant Extract against HSV-1</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">HSV-1</style></keyword><keyword><style  face="normal" font="default" size="100%">Natural product activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Simple cell-based screening</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">251-259</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Backgrounds: &lt;/strong&gt;Drug screening is a time-consuming and costly process confronted with low productivity and challenges in using animals, which limits the discovery of new drugs. The cellbased assay allows the minimization of using the animal models and can provide more relevant &lt;em&gt;in vivo &lt;/em&gt;biological information than biochemical assay. &lt;strong&gt;Objective:&lt;/strong&gt; We aimed to establish a simple cell-based screening assay for the discovery of lead extract against HSV-1. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Assay setting up was performed by optimization of the cell, incubation time, virus titer, and determination of Z value. &lt;strong&gt;Results: &lt;/strong&gt;We have successfully established reproducible methods, by setting up assay plate including determination: 1) Vero cells as a model for HSV-1 infection, 2) Incubation for 5 days as sufficient time for CPE endpoint at monolayer cells, 3) 100 TCID&lt;sub&gt;50&lt;/sub&gt;/well HSV-1 as infection titer which caused high percentage of cell detachment, 4) determination of Z value of 100 TCID&lt;sub&gt;50&lt;/sub&gt;/well infection &amp;gt; 0.5. In addition, the established system was tested using ACV as the most common anti-HSV drug. Furthermore, we demonstrated the current system to screen extracts from &lt;em&gt;Acacia nilotica, Uncaria gambir &lt;/em&gt;and &lt;em&gt;Aspalathus linearis &lt;/em&gt;against HSV-1. It was observed that the alkaline extract of Uncaria gambir exhibited the highest SI (12.5) compared to other extracts. &lt;strong&gt;Conclusion: &lt;/strong&gt;We demonstrated current cellbased screening system was reproducible and able to identify lead extracts against HSV-1 infection.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">251</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ratika Rahmasari&lt;sup&gt;1&lt;/sup&gt;, Takahiro Haruyama&lt;sup&gt;2&lt;/sup&gt;, Muhareva Raekiansyah&lt;sup&gt;3&lt;/sup&gt;, Farhana Mossadeque&lt;sup&gt;4&lt;/sup&gt;, Marina Ika Irianti&lt;sup&gt;1&lt;/sup&gt;, Ayun Erwina Arifianti&lt;sup&gt;1&lt;/sup&gt;, Nobuyuki Kobayashi&lt;sup&gt;2,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Central Research Center, AVSS, Okinawa, JAPAN.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;PT Sciencewerke Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Institute of Tropical Medicine, Nagasaki University, Nagasaki, JAPAN.&lt;/p&gt;
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