<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ajayi Adebimpe Oluwabunmi</style></author><author><style face="normal" font="default" size="100%">Aruwa Christiana Eleojo</style></author><author><style face="normal" font="default" size="100%">Sabiu Saheed</style></author><author><style face="normal" font="default" size="100%">Akinyosoye Felix Akinsola</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cheminformatic and in vitro Bioprospection of Capsicum Annuum L. Metabolites as DNA Gyrase B Inhibitors</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Capsicum annuum</style></keyword><keyword><style  face="normal" font="default" size="100%">Computational analysis.</style></keyword><keyword><style  face="normal" font="default" size="100%">Gyrase B</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemicals</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">726-737</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Capsicum species are known in food and trado-medicinal uses for maladies management due their rich content of phytochemicals, but with little work done on &lt;em&gt;in silico&lt;/em&gt; bioprospection of its volatilome. Objectives: This study targeted chemometric profiling, virtual bioprospection of potential lead metabolites in 2 &lt;em&gt;Capsicum annuum&lt;/em&gt; L. fruit variants’ (green and red) to identify lead gyrase B inhibitors (GBIs) and provide new mechanistic insights. &lt;strong&gt;Methods: &lt;/strong&gt;Metabolites were profiled using Gas Chromatography-Mass Spectrometry (GC-MS), and quantitative phytochemical assays. Extracts antioxidant (DPPH, ABTS, FRAP) and antibacterial (susceptibility testing) activities were also determined.&lt;em&gt; In silico&lt;/em&gt; [docking, pharmacokinetics, DFT] analyses were used to identify and predict chemical features of potential lead GBIs key to extracts molecular mechanism of action. &lt;strong&gt;Results: &lt;/strong&gt;Mass spectral analysis identified hydrocarbons, fatty acid and other derivatives. Quantitative phytochemical analysis showed flavonoids, cardiac glycosides and alkaloids. The green&lt;em&gt; C. annuum&lt;/em&gt; extract had better antioxidative action, while extracts of both green and red variant showed similar antimicrobial profiles against resistant bacterial pathogens. &lt;em&gt;In silico &lt;/em&gt;highest docking scores were observed for [1-Ethyloctyl) cyclohexane (-6.6 kcal/mol)] and dibutyl phthalate (-6.4 kcal/mol). All lead GBIs had desirable pharmacokinetics in line with the Lipinski rule of 5, and chemical reactivity properties. &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;In silico&lt;/em&gt; and &lt;em&gt;in vitro &lt;/em&gt;methods combination provided robust metabolomic profiling. The identified lead &lt;em&gt;C. &lt;/em&gt;&lt;em&gt;annuum&lt;/em&gt;-based natural GBIs contribute to the bioactivity profile and molecular mechanism of action of fractions. The study provided a first-hand report on natural GBIs derivable from Capsicum fruits which could be exploited in formulations for non-food and pharmaceutical applications.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">726</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ajayi Adebimpe Oluwabunmi&lt;sup&gt;1&lt;/sup&gt;, Aruwa Christiana Eleojo&lt;sup&gt;2*&lt;/sup&gt;, Sabiu Saheed&lt;sup&gt;2&lt;/sup&gt;, Akinyosoye Felix Akinsola&lt;sup&gt;1 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Microbiology, School of Life Sciences, Federal University of Technology,&amp;nbsp;Akure, PMB 704, Ondo State, NIGERIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, PO Box 1334, Durban 4000, SOUTH AFRICA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mayowa Oladele Agunbiade</style></author><author><style face="normal" font="default" size="100%">Sabiu Saheed</style></author><author><style face="normal" font="default" size="100%">Esta Van Heerden</style></author><author><style face="normal" font="default" size="100%">Carolina Henritta Pohl</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vivo Toxicopathological Evaluation of a Purified Bioflocculant Produced by Arthrobacter humicola</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Actinomycetes</style></keyword><keyword><style  face="normal" font="default" size="100%">Arthrobacter humicola</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioflocculant</style></keyword><keyword><style  face="normal" font="default" size="100%">Hematological</style></keyword><keyword><style  face="normal" font="default" size="100%">Polysaccharides</style></keyword><keyword><style  face="normal" font="default" size="100%">Wastewater treatment</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">486-492</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; In spite of the commendable flocculating activities of microbial flocculants, a good number of them potentiate significant toxicity. This study evaluated the &lt;em&gt;in vivo&lt;/em&gt; toxicological implications of treatment with the Purified bioflocculant (PB) from &lt;em&gt;Arthrobacter humicola&lt;/em&gt; using OECD guidelines. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; In the acute toxicity assessment, a single oral administration of 2000 mg/kg body weight (b.w.) of PB was given to the Wistar rats and the animals were observed for 2 weeks. The daily dose toxicity testing was performed through daily oral treatment with graded doses (50, 200 and 500 mg/kg b.w.) of PB for 4 weeks. Clinical signs of toxicity, behavioral changes, hematological and biochemical parameters were thereafter evaluated. &lt;strong&gt;Results:&lt;/strong&gt; PB at 2000 mg/kg b.w. produced no treatment-mediated signs of toxicity, behavioral changes or mortality in the animals. Thus, its no-observed-adverse-effect level was estimated to be above 2000 mg/kg b.w. In the repeated dose toxicity testing, treatments with PB also revealed no significant differences in the feeding patterns, lipid profiles, hematological and clinical biochemistry parameters when compared with the control group. Although, at 500 mg/kg b.w. PB, a significant increase was observed in the serum activities of alkaline phosphatase, nonetheless, cage side observations recorded no treatment-induced signs of toxicity and macro-histopathological examinations of all the investigated organs also revealed no obvious morphological changes. &lt;strong&gt;Conclusion:&lt;/strong&gt; The overall results suggested that PB was well tolerated by the animals and is endowed with monosaccharides bearing functional groups of flocculation importance, thus, suggesting its potential application as a safe actinomycetes bioflocculant for water treatment.&amp;nbsp;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">486</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Mayowa Oladele Agunbiade&lt;sup&gt;1,2*&lt;/sup&gt;, Sabiu Saheed&lt;sup&gt;1,3&lt;/sup&gt;, Esta Van Heerden&lt;sup&gt;1,4&lt;/sup&gt;, Carolina Henritta Pohl&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, 9301, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biocatalysis and Technical Biology Research Group, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Applied Sciences, Durban University of Technology, SOUTH AFRICA 4iWater Pyt Limited, Walter Sisulu 5, Bloemfontein, SOUTH AFRICA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Aladodo Abimbola Raliat</style></author><author><style face="normal" font="default" size="100%">Sabiu Saheed</style></author><author><style face="normal" font="default" size="100%">Sulyman Olarewaju Abdulhakeem</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pteleopsis suberosa Engl. and Diels (Combretaceae) aqueous stem bark extract extenuates oxidative damage in streptozotocin-induced diabetic Wistar rats</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Arjunglycoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Diabetes</style></keyword><keyword><style  face="normal" font="default" size="100%">Glucose-6-phosphate dehydrogenase</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyperglycaemia</style></keyword><keyword><style  face="normal" font="default" size="100%">Pteleopsis suberosa</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">183-190</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;strong&gt;Context&lt;/strong&gt;: Pteleopsis suberosa Engl. and Diels (Combretaceae) is an ethnomedicinally valued deciduous shrub with significant therapeutic application against diabetes in West Africa. Aims: This study evaluated the hypoglycaemic and antioxidant activity of its aqueous stem bark extract (PSAE) over a 15-day experimental period in streptozotocin (STZ)-challenged diabetic rats.&lt;strong&gt; Methods and Materia&lt;/strong&gt;l: STZ-induced diabetic rats were administered 50, 100 and 200 mg/kg body weight of the extract once daily and the blood glucose levels were monitored for 15 days. The bioactive constituents of PSAE were analyzed using Gas Chromatography- Mass Spectrometry (GC-MS) technique. &lt;strong&gt;Results&lt;/strong&gt;: Treatment with PSAE markedly (&lt;em&gt;p&amp;lt;&lt;/em&gt; 0.05) ameliorated the concentrations of blood glucose and improved the lipid metabolism imbalance in the diabetic animals. Also, while PSAE significantly (&lt;em&gt;p&amp;lt;&lt;/em&gt;0.05) improved glycogen and glutathione (reduced) levels as well as glucose-6-phosphate dehydrogenase, superoxide dismutase and catalase activities, the concentrations of glucose and malondialdehyde coupled with the activity of α-amylase were significantly reduced in the liver of PSAE-administered diabetic animals when compared with those of the normal control and untreated diabetic groups. The observed improvements could be associated with the phytoconstituents of PSAE as evident from the results of the GC-MS analysis. Put together, the elicited effects compared favorably well with metformin (a standard hypoglycemic drug) and are suggestive of antidiabetic and antioxidant capabilities of the extract. &lt;strong&gt;Conclusion&lt;/strong&gt;: The scope of the data presented in this study indicates that the extract ameliorated streptozotocin-induced diabetes in rats and it is suggested to be mediated via antioxidant and concerted blood glucose lowering actions.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">183</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Aladodo Abimbola Raliat&lt;sup&gt;2&lt;/sup&gt;, Sabiu Saheed&lt;sup&gt;1,&lt;/sup&gt;*, Sulyman Olarewaju Abdulhakeem&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Nelson Mandela Drive, 9301 Bloemfontein, SOUTH AFRICA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Biochemistry and Pharmacology, School of Basic Medical Sciences, Kwara State University, Malete. P.M.B. 1530, Ilorin, NIGERIA.&lt;/p&gt;
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