<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Manab Mandal</style></author><author><style face="normal" font="default" size="100%">Debabrata Misra</style></author><author><style face="normal" font="default" size="100%">Narendra Nath Ghosh</style></author><author><style face="normal" font="default" size="100%">Sukhendu Mandal</style></author><author><style face="normal" font="default" size="100%">Vivekananda Mandal</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GC-MS Analysis of Anti-Enterobacterial Dichloromethane Fraction of Mandukaparni (Hydrocotyle javanica Thunb.) – A plant from Ayurveda</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-enterobacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemical profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Mandukaparni</style></keyword><keyword><style  face="normal" font="default" size="100%">Time kill assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Volatile oils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1494-1503</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Mandukaparni (&lt;em&gt;Hydrocotyle javanica&lt;/em&gt; Thunb.) is a well known medicinal herb used as folklore medcine in many chronic and infectious gastric and other diseases by the people of Estern Himalayan regions. However, the therapeutic active principles of this plant remained unknown. &lt;strong&gt;Objective:&lt;/strong&gt; The main objective of the study was to characterize antienterobacterial dichloromethane fraction of the volatile oils of it by GC-MS. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; In the present study, dichloromethane (DCM) fraction (MP-DCMf) of Mandukaparni was collected by phase separation of the methanol extract and tested for anti-enterobacterial potentiality against human pathogenic gastrointestinal and food poisoning bacteria by agar well diffusion assay, viability assay and LDH assay and SEM studies. Characterization of the active MP-DCMf fraction was performed by TLC and GC-MS analysis.&lt;strong&gt; Results: &lt;/strong&gt;The MP-DCMf possessed bio-active compounds that have antibacterial potentiality against both the Grampositive and Gram-negative bacteria. The MIC and MBC values were in the range from 1.56 mg/ml to 0.78 mg/ml and 6.25 to 1.56 mg/ml, respectively. The time kill assay showed that at a dose of 3.12 mg/ml of MP-DCMf was lethal to the &lt;em&gt;E. coli&lt;/em&gt; MTCC 723 at the 18th hr of treatment. LDH release had moderate positive correlation with the activity index and time of treatment whereas strong negative correlation with CFU count. It caused highest cell disruption in &lt;em&gt;S. mutans.&lt;/em&gt; The principal compounds were D-carvon (30.949%);1H-Isoindole-1,3(2H)-dione; 2-(2-chlorophenyl) (28.483%);Cyclohexanone; 2-methyl-5-(1-methylethen) trans (10.04%); D-Limnone (9.256%);2,6-Octadien-1-ol, 3,7-dimethyl- acetate (6.684%); p-Cresol (0.551%), and Thymol (0.118%). Pub-chem database search also supports that these compounds have very strong bactericidal activity by membrane damage as evidenced by LDH and SEM studies. &lt;strong&gt;Conclusions:&lt;/strong&gt; MP-DCMf contains many potential antibacterial compounds that can be used to combat the gastrointestinal and food poising bacterial pathogens.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1494</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Manab Mandal&lt;sup&gt;1&lt;/sup&gt;, Debabrata Misra&lt;sup&gt;1&lt;/sup&gt;, Narendra Nath Ghosh&lt;sup&gt;1&lt;/sup&gt;, Sukhendu Mandal&lt;sup&gt;2&lt;/sup&gt;, Vivekananda Mandal&lt;sup&gt;1&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;University of Gour Banga, Malda, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;2University of Calcutta, Calcutta, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Debabrata Misra</style></author><author><style face="normal" font="default" size="100%">Manab Mandal</style></author><author><style face="normal" font="default" size="100%">Narendra Nath Ghosh</style></author><author><style face="normal" font="default" size="100%">Vivekananda Mandal</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pharmacognostic Standardization of an Ethnomedicinal Aquatic Herb, Monochoria hastata (L.) Solms for its Antibacterial Potentiality</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Fluorescence characteristics</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metal accumulation</style></keyword><keyword><style  face="normal" font="default" size="100%">M hastata (L.) solms</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacognostic standardization</style></keyword><keyword><style  face="normal" font="default" size="100%">Traditional medicinal plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/520</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">533-540</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; To evaluate antibacterial potentiality, pharmacognostic characteristics and quality control parameters including heavy metals, like lead (Pb) and arsenic (As) accumulation in the aerial parts of an aquatic herb, &lt;em&gt;Monochoria hastata&lt;/em&gt; (L.) Solms. &lt;strong&gt;Methods:&lt;/strong&gt; Antibacterial assay was done by agar well diffusion method. Pharmacognostic studies like morpho-anatomical and physicochemical analyses were carried out for organoleptic, microscopic and macroscopic evaluations of living aerial parts, and powder microscopy, fluorescence, proximate and elemental analysis of the dried powder sample. Elements and heavy metals contents were determined by carbon, hydrogen, nitrogen, sulphur / oxygen (CHNS/O) analyzer and inductively coupled plasma mass spectrometry (ICP-MS), respectively. &lt;strong&gt;Results:&lt;/strong&gt; &lt;em&gt;M. hastata &lt;/em&gt;(L.) Solms aerial parts showed antibacterial activity against gastrointestinal and topical pathogens. It exhibited an amphistomatic and hydromorphic anatomical characters. The distinguishing features were the presence of stomata in upper and lower epidermis, broad air chambers, cuticle, collateral vascular bundles, sclereidal fibres, pitted tracheids, pitted vessels, calcium oxalate crystals and annular vessels in leaf. The powder sample contained very less amount of acid insoluble ash than water soluble ash and complete absence of foreign organic matter. Carbon, hydrogen, nitrogen and sulfur (CHNS) ratio was 33:6:5:1 and lead content was lesser than the recommended upper limit though the arsenic content was higher than the permissible upper limit. &lt;strong&gt;Conclusions:&lt;/strong&gt; Though the plant has traditionally been used as a potent Ethnomedicinal herb to cure boils, gastritis, hepatopathy and as laxative, but no such evaluation of pharmacognostic identity and quality parameters have been done so far. This is the first report on its pharmacognostic characters and quality control issues like heavy metal accumulation and physicochemical parameters for future use as powder drug.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">533</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Debabrata Misra&lt;sup&gt;1&lt;/sup&gt;, Manab Mandal&lt;sup&gt;1&lt;/sup&gt;, Narendra Nath Ghosh&lt;sup&gt;2&lt;/sup&gt;, Vivekananda Mandal&lt;sup&gt;1*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Plant and Microbial Physiology and Biochemistry Laboratory, Department of Botany, University of Gour Banga, Malda, West Bengal, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, University of Gour Banga, Malda, West Bengal, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>