<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sajad Hassan Wani</style></author><author><style face="normal" font="default" size="100%">Hilal Ahmad Bhat</style></author><author><style face="normal" font="default" size="100%">Javid Iqbal Mir</style></author><author><style face="normal" font="default" size="100%">Shahid Ali Akbar</style></author><author><style face="normal" font="default" size="100%">Sajad Un Nabi</style></author><author><style face="normal" font="default" size="100%">Desh Beer Singh</style></author><author><style face="normal" font="default" size="100%">Nazeer Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bio efficacy</style></keyword><keyword><style  face="normal" font="default" size="100%">Irigenin</style></keyword><keyword><style  face="normal" font="default" size="100%">Iris Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">RP-HPLC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/377</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">s23-s27</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;Irigenin belongs to family of &lt;em&gt;isoflavonoids&lt;/em&gt;, isolated from &lt;em&gt;Iris Plant&lt;/em&gt; of Kashmir Himalaya. A rapid and specific reverse phase high pressure chromatography (RP HPLC) method for quantitative analysis of irigenin in the different species of &lt;em&gt;Iris Plant&lt;/em&gt; was developed. The samples were analyzed on RP-C&lt;sub&gt;18&lt;/sub&gt; e column (chromolith, 5&amp;mu;m, 4.6&amp;times;100 mm). The HPLC system was operated at ambient temperature (&amp;plusmn;30c). The mobile phase consisted of methanol: water. The detecting wavelength at 260 nm and flow rate of 0.6 ml/min. The standard irigenin was diluted using the mobile phase at a known concentration of 1mg/ml; the sample was filtered through sample filter of 0.45 &amp;mu; pore size. The filtrate was introduced on to a reverse phase analytical column. The content of irigenin in the different species of &lt;em&gt;Iris Plant&lt;/em&gt; was determined. The HPLC showed an excellent performance in separating the irigenin in different species of &lt;em&gt;Iris Plant&lt;/em&gt;. Furthermore, the antipathogenic activity. The test compound at each respective concentration was found to be statistically superior against scab. Furthermore, the test compound @ 5000 ppm proved significantly most effective by providing (82.49%) inhibition in the mycelia growth of apple scab. It was followed by fusarium (77.27%) at 5000 ppm. Lowest reduction in mycelia growth (65.78%) was recorded in &lt;em&gt;marssonina&lt;/em&gt; and did not differ significantly from Alternaria (67.47%) at 5000 ppm. Furthermore, lowest inhibition of mycelia growth was recorded at 1000 ppm. Similar trend was recorded for rest of the pathogens i.e. highest reduction at 5000 ppm, lowest at 1000 ppm and at 2000, 3000 and 4000 ppm it ranges between the first two but increases with increase in concentration. From this study irigenin is potent compound which can be used for controlling the growth of respective pathogens.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">s23</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sajad Hassan Wani&lt;sup&gt;1&lt;/sup&gt;*, Hilal Ahmad Bhat&lt;sup&gt;1&lt;/sup&gt;, Javid Iqbal Mir&lt;sup&gt;1&lt;/sup&gt;, Shahid Ali Akbar&lt;sup&gt;2&lt;/sup&gt;, Sajad un Nabi&lt;sup&gt;3&lt;/sup&gt;, Desh Beer Singh&lt;sup&gt;4&lt;/sup&gt;, Nazeer Ahmad&lt;sup&gt;5 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Biotechnology division, Central Institute of Temperate Horticulture- ICAR, Srinagar, Jammu and Kashmir, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Entomology division, Central Institute of Temperate Horticulture- ICAR, Srinagar, Jammu and Kashmir, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Plant Pathology, Central Institute of Temperate Horticulture- ICAR, Srinagar, Jammu and Kashmir, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Central Institute of Temperate Horticulture- ICAR, Srinagar, Jammu and Kashmir, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Sheri Kashmir University of Agriculture Science and Technology Kashmir (SKUAST-K), Jammu, Jammu and Kashmir, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>