<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sangeetha Muniaraj</style></author><author><style face="normal" font="default" size="100%">Vijayakumar Subramanian</style></author><author><style face="normal" font="default" size="100%">Prabhu Srinivasan</style></author><author><style face="normal" font="default" size="100%">Manogar Palani</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In silico and in vitro Studies on Lyngbya majuscula using against Lung Cancer Cell Line (A549)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Lyngbya majuscula</style></keyword><keyword><style  face="normal" font="default" size="100%">Lyngbyastatin</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT assay DAPI staining</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/502</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">421-428</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; To predict an anticancer drug from the members of cyanobacteria,&lt;em&gt; in silico&lt;/em&gt; molecular docking was carried out between the cyanobacterial bioactive compounds and lung cancer causing receptor. The highest docking score was produced by Lyngbyastatin (&lt;em&gt;Lyngbya majuscula&lt;/em&gt;). In the present study anticancer potential of &lt;em&gt;L. majuscula&lt;/em&gt; was evaluated on human lung cancer cell line (A549) using its methanolic extract. &lt;strong&gt;Methods:&lt;/strong&gt; Molecular docking was carried out between the Epidermal Growth Factor Receptor tyrosine kinase and cyanobacterial compounds. Based on the docking results, Lyngbyastatin was found to be the most effective compound. As this compound is present in the &lt;em&gt;L. majuscula,&lt;/em&gt; the cytotoxicity of this organism was assessed by standard cell viability assays like MTT method. Algal methanolic extract treated with A 549 cell line morphology was studied by DAPI staining. DNA fragmentation assay was also conducted to study the presence of DNA laddering. &lt;strong&gt;Results:&lt;/strong&gt; Totally 75 bioactive compounds were docked with Epidermal Growth Factor Receptor tyrosine kinase . Of them, 12 compounds were selected based on the docking score. Among the 12 bioactive compounds, Lyngbyastatin found to be most effective compound. &lt;em&gt;L. majuscula&lt;/em&gt; showed potential anticancer activity against A549 cell line with IC&lt;sub&gt;50&lt;/sub&gt; value of 14.82&amp;plusmn; 0.62 &amp;mu;g/ml in MTT method. Most of the treated cells lost their characteristic stretched appearance with shrinkage of nucleus. DNA profile revealed the presence of sheared DNA in treated ones but no fragmentation was observed. &lt;strong&gt;Conclusion:&lt;/strong&gt; The results indicated potent anticancer of algal methanolic extract on A549 cell line, which may be good candidates for further investigation to isolate bioactive anticancer compounds.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">421</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sangeetha Muniaraj&lt;sup&gt;1&lt;/sup&gt;, Vijayakumar Subramanian&lt;sup&gt;2*&lt;/sup&gt;, Prabhu Srinivasan&lt;sup&gt;2&lt;/sup&gt;, Manogar Palani&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Microbiology, Kamaraj College, Tuticorin-628003, Tamil Nadu, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;PG and Research, Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College, Poondi, Thanjavur-613503, Tamil Nadu, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ramesh Venkadachalam</style></author><author><style face="normal" font="default" size="100%">Vijayakumar Subramaniyan</style></author><author><style face="normal" font="default" size="100%">Manogar Palani</style></author><author><style face="normal" font="default" size="100%">Mahadevan Subramaniyan</style></author><author><style face="normal" font="default" size="100%">Prabhu Srinivasan</style></author><author><style face="normal" font="default" size="100%">Murugan Raji</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mosquito Larvicidal and Pupicidal Activity of Tephrosia purpurea Linn. (Family: Fabaceae) and Bacillus sphaericus against, Dengue Vector, Aedes aegypti</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Aedes aegypti</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacillus sphaericus</style></keyword><keyword><style  face="normal" font="default" size="100%">Dengue vector</style></keyword><keyword><style  face="normal" font="default" size="100%">Larvicidal activity.</style></keyword><keyword><style  face="normal" font="default" size="100%">Tephrosia purpurea</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/169</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">737-742</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; The bio-efficacy of Tephrosia purpurea leaf extract and bacterial insecticide, &lt;em&gt;Bacillus sphaericus&lt;/em&gt; larvicidal activity was assessed against the first to fourth instars larvae of &lt;em&gt;Aedes aegypti&lt;/em&gt;, under the laboratory conditions. &lt;strong&gt;Methods:&lt;/strong&gt; The plant material was shade dried at room temperature and powdered coarsely. &lt;em&gt;T. purpurea and B. sphaericus&lt;/em&gt; show the various concentrations of larvicidal and pupicidal activity against various instars larvae of &lt;em&gt;A. aegypti.&lt;/em&gt; &lt;strong&gt;Results:&lt;/strong&gt; The LC&lt;sub&gt;50&lt;/sub&gt; of &lt;em&gt;T. purpurea&lt;/em&gt; against the first to fourth instars larvae were 139.24, 176.24, 219.28, 256.27, and 326.29 ppm and the 480.72, 541.21, 580.34, 672.20, and 762.80 ppm, respectively. &lt;em&gt;B. sphaericus&lt;/em&gt; against the first to fourth instars larvae the LC&lt;sub&gt;50&lt;/sub&gt; values were 46.16, 56.23, 69.82, 80.81 and 96.12 ppm and the LC&lt;sub&gt;90&lt;/sub&gt; values 141.68, 172.46, 184.21, 193.31 and 218.16 ppm, respectively. However, the combined treatment of &lt;em&gt;T. purpurea + B. sphaericus&lt;/em&gt; (1:2) material shows highest larvicidal activity of the LC&lt;sub&gt;50&lt;/sub&gt; values 80.08, 82.21, 88.00, 92.21 and 98.16 ppm; The LC&lt;sub&gt;90&lt;/sub&gt; values of 108.39, 118.71, 136.75, 149.02 and 153.24 ppm, against &lt;em&gt;A. aegypti&lt;/em&gt; in all the tested concentrations than the individuals and clearly established that there is a substantial amount of synergist act. &lt;strong&gt;Conclusion:&lt;/strong&gt; The present study reported that both &lt;em&gt;T. purpurea and B. sphaericus&lt;/em&gt; materials could serve as a potential larvicidal agent. Since, &lt;em&gt;A. aegypti&lt;/em&gt; is a container breeder vector mosquito this user and eco-friendly and low-cost vector control strategy could be a viable solution to the existing dengue disease burden. Therefore, this study provides first report on the mosquito larvicidal activity the combined effect of &lt;em&gt;T. purpurea&lt;/em&gt; leaf extract and &lt;em&gt;B. sphaericus&lt;/em&gt; against as target species of &lt;em&gt;A. aegypti&lt;/em&gt;.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">737</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Ramesh Venkadachalam&lt;sup&gt;1&lt;/sup&gt;, Vijayakumar Subramaniyan&lt;sup&gt;1&lt;/sup&gt;, Manogar Palani&lt;sup&gt;1&lt;/sup&gt;, Mahadevan Subramaniyan&lt;sup&gt;1&lt;/sup&gt;, Prabhu Srinivasan&lt;sup&gt;1&lt;/sup&gt; and Murugan Raji&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;P.G. and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur district, Tamil Nadu, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Botany Govt Arts College (Autonomous), Kumbakonam 612002. Tamil Nadu, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>