<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bosco Lawarence</style></author><author><style face="normal" font="default" size="100%">Murugan K</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comprehensive Evaluation of Antioxidant Potential of Selected Osbeckia species and their in vitro Culture, Purification and Fractionation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anthocyanins</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant Capacity</style></keyword><keyword><style  face="normal" font="default" size="100%">Free Radicals</style></keyword><keyword><style  face="normal" font="default" size="100%">Osbeckia Spp.</style></keyword><keyword><style  face="normal" font="default" size="100%">Reactive Oxygen Species</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/pj-9-5/10.5530pj.2017.5.107/index.html</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">674-682</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Health-benefit properties of natural pigments have been intensely studied, especially the anthocyanins. In the last few decades, research on anthocyanins has attracted biologists by the increasing evidence of their health beneficial effects. &lt;em&gt;Osbeckia,&lt;/em&gt; belongs to Melastomataceae and is well-known for colouring pigments and other bioactive compounds. In the present study, total anthocyanin and antioxidant capacity indicators were evaluated from 8 &lt;em&gt;Osbeckia&lt;/em&gt; spp. and anthocyanin was extracted from &lt;em&gt;in vitro&lt;/em&gt; cultures of &lt;em&gt;O. aspera&lt;/em&gt; and &lt;em&gt;O. reticulata&lt;/em&gt;. Materials and &lt;strong&gt;Methods:&lt;/strong&gt; The antioxidant effect was studied using ABTS (2, 2&amp;rsquo;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid) radical cation decolourisation assay, the FRAP, the scavenging ability of hydroxyl radicals and the superoxide anion scavenging activity. Anthocyanin extracted from &lt;em&gt;in vitro&lt;/em&gt; cultures were purified and fractionated using column chromatography and LC-MS MS analysis. &lt;strong&gt;Results:&lt;/strong&gt; &lt;em&gt;In vitro&lt;/em&gt; cultures of &lt;em&gt;O. aspera&lt;/em&gt; was obtained in MS medium fortified with various combinations of Benzyl Adenine (BA), Naphthalene acetic acid (NAA) and 2, 4-D. The chromatograms of &lt;em&gt;O. aspera&lt;/em&gt; revealed the presence of malvidin-3 -diglucoside, peonidin, delphinidin and cyanindin whereas &lt;em&gt;O. reticulata &lt;/em&gt;cultures accumulated large amounts of malvidin, cyanindin and cyanidin aglycone. The purified anthocyanins of these species were evaluated for their antioxidant potential and was found more remarkable than the crude extracts. &lt;strong&gt;Conclusion&lt;/strong&gt;: &lt;em&gt;Osbeckia&lt;/em&gt; species are rich in anthocyanin and therefore display potential AOX power. &lt;em&gt;O. aspera&lt;/em&gt; and &lt;em&gt;O. reticulata&lt;/em&gt; callus was induced &lt;em&gt;in vitro&lt;/em&gt; production of anthocyanins. The pool of anthocyanins was purified and fractionated by LCMS/ MS and AOX assays were performed with the purified anthocyanin which showed higher level activities.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">674</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Bosco Lawarence and Murugan K &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Plant Biochemistry and Molecular Biology Laboratory, University College, Trivandrum, Kerala, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>