<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Farah J. Hashim</style></author><author><style face="normal" font="default" size="100%">Sukrita Punyauppa-Path</style></author><author><style face="normal" font="default" size="100%">Warayutt Pilap</style></author><author><style face="normal" font="default" size="100%">Jaehong Han</style></author><author><style face="normal" font="default" size="100%">Nattawadee Kanpipit</style></author><author><style face="normal" font="default" size="100%">Pongpat Kiatprasert</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic and DNA-Damaging Effects of Curcuminoids on Daudi and Jurkat Leukemic Cell Lines: Investigating Potential Alkylating Activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer; Apoptosis; Alkyl group; DNA fragmentation; Dapi Stain</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2026</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2026</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">339-347</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;This study aimed to assess novel role of curcuminoids as an alkylating agent against leukemic cells (Daudi and Jurkat cell lines). Cytotoxic activity was assessed using an MTT assay. Also, 4-(4’-nitrobenzyl) pyridine (NBP) was used as a DNA model for in vitro DNA alkylation. Results showed curcuminoids possess cytotoxic activity against both the Daudi and Jurkat cell lines. They showed 21.44 μg/ml and 48.82 μg/ ml IC50 values, compared to treatment with Melphalan at 22.37 μg/ml and 56.51 μg/ml for the Daudi and Jurkat cell lines, respectively. The curcuminoids were found to possess some alkylating activity, similar to that of Melphalan. In conclusion, chemical screening revealed an intrinsic property of curcuminoids with regard to their in vitro alkylating-like effects; however, these chemicals exert their cytotoxic functions on Daudi and Jurkat cells through a combination of DNA injury mechanisms. This indicates that alkylating-like chemical properties may act synergistically with other forms of cellular stress in activating the process of apoptosis, which expands the understanding of curcuminoids in treating hematological malignancies.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">339</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Farah J. Hashim&lt;sup&gt;1*&lt;/sup&gt;, Sukrita Punyauppa-Path&lt;sup&gt;2&lt;/sup&gt;, Warayutt Pilap&lt;sup&gt;3,4&lt;/sup&gt;, Jaehong Han&lt;sup&gt;5&lt;/sup&gt;, Nattawadee Kanpipit&lt;sup&gt;6&lt;/sup&gt;, Pongpat Kiatprasert&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biology, College of Science, Baghdad University, Baghdad, IRAQ.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Mathematics and Science, Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin 32000, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Walai Rukhavej Botanical Research Institute, Mahasarakham University, Mahasarakham, 44150, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Center of Excellence in Biodiversity Research, Mahasarakham University, Mahasarakham, 44150, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Metalloenzyme Research Group and Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, KOREA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, THAILAND.&lt;/p&gt;
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