<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ekta Panchal</style></author><author><style face="normal" font="default" size="100%">Shiv Kumar Yadav</style></author><author><style face="normal" font="default" size="100%">Neha Jain</style></author><author><style face="normal" font="default" size="100%">Mahima Chauhan</style></author><author><style face="normal" font="default" size="100%">Deepika Yadav</style></author><author><style face="normal" font="default" size="100%">Archana Sharma</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Reversed-Phase- HPLC Method Development and Validation for Simultaneous Determination of Dexamethasone and Curcumin in Bulk Mixtures</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Curcumin</style></keyword><keyword><style  face="normal" font="default" size="100%">Dexamethasone</style></keyword><keyword><style  face="normal" font="default" size="100%">Qualitative</style></keyword><keyword><style  face="normal" font="default" size="100%">Quantitative</style></keyword><keyword><style  face="normal" font="default" size="100%">RP-HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Validation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2026</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2026</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">221-228</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;The combination of curcumin and dexamethasone can improve anti-inflammatory benefits, lower the dosage of corticosteroids needed, and offer a more comprehensive therapeutic strategy with fewer side effects in treating rheumatoid arthritis. &lt;strong&gt;Objective:&lt;/strong&gt; This research work aimed to develop a specific and reproducible RP (Reversed-Phase)–HPLC analytical method with a PDA detector for the qualitative and quantitative determination of dexamethasone and curcumin in a bulk mixture. &lt;strong&gt;Methods:&lt;/strong&gt; The RP technique was employed in which the HPLC instrument was equipped with a PDA detector and a C18 analytical column (250 × 4.6 mm, 5μm) at 30°C temperature. The mobile phase used in this work consisting 0.1 % Orthophosphoricacid: Acetonitrile at the specified ratio of 30:70 accompanied by a defined flowrate of 1mL/min with an injectionvolume of 10μL. The spectrum detected for the diluted simple solution at wavelength of 280nm using UV visible spectroscopy for good resolution. &lt;strong&gt;Results:&lt;/strong&gt; This method resulted in two distinct peaks of dexamethasone and curcumin with a retention time of 3.16 and 4.16minutes respectively and all studied parameters which ensure the suitability of the developed method were analyzed and fall within the aceptable criteria. The sensitivity was detected at 0.232μg/ml &amp;amp; 0.704μg/ ml, respectively, for dexamethasone while, for curcumin it was found to be 0.065μg/ml and 0.198μg/ml, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; The validation methodology was developed by ICH guidelines for its linearity, accuracy, precision, robustness, LOD, &amp;amp; LOQ. From the results, it can be concluded that the developed method was found to be satisfactory in terms of reproducibility and accuracy. The chromatogram shows two distinct peaks for both drugs and further, it could be effectively utilized in the quantitative and qualitative estimation of both drugs for any pharmaceutical research.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">221</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Ekta Panchal&lt;sup&gt;1, 2&lt;/sup&gt;, Shiv Kumar Yadav&lt;sup&gt;2*&lt;/sup&gt;, Neha Jain&lt;sup&gt;1&lt;/sup&gt;, Mahima Chauhan&lt;sup&gt;3&lt;/sup&gt;, Deepika Yadav&lt;sup&gt;2&lt;/sup&gt;, Archana Sharma&lt;sup&gt;1*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Amity Institute of Pharmacy, Amity University Uttar Pradesh, Sector 125, Noida 201303, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Anangpuria School of Pharmaceutical Sciences, Pt. B. D. Sharma University of Health Sciences, Alampur, Faridabad121004, Haryana, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;HIMT College of Pharmacy College, Greater Noida, Uttar Pradesh, 201301, INDIA.&lt;/p&gt;
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