<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Puspa SD. Solihah</style></author><author><style face="normal" font="default" size="100%">Ellisa Clara Pasaribu</style></author><author><style face="normal" font="default" size="100%">Fahmi Ahsanul Haq</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Network Pharmacology-based Prediction and In Vivo Validation of Red Ginger and Turmeric Extract Combinations against Hypercoagulability in Isoproterenol-Induced Rats</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticoagulant</style></keyword><keyword><style  face="normal" font="default" size="100%">Coagulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Curcuma longa</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoproterenol</style></keyword><keyword><style  face="normal" font="default" size="100%">Network pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Zingiber officinale rubrum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2026</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2026</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">139-149</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Myocardial infarction is a critical cardiovascular condition often preceded by a hypercoagulable state. Red ginger (&lt;em&gt;Zingiber officinale&lt;/em&gt; var. &lt;em&gt;rubrum&lt;/em&gt;, ZOR) and turmeric (&lt;em&gt;Curcuma longa&lt;/em&gt; L., CL) are recognized for their anticoagulant properties, yet their multi-target pharmacological interactions remain poorly understood. This study aimed to evaluate the anticoagulant potential of ZOR and CL combinations using an integrated network pharmacology and&lt;em&gt; in vivo&lt;/em&gt; approach. &lt;strong&gt;Methods: &lt;/strong&gt;Network pharmacology analysis was employed to predict potential molecular targets of ZOR and CL bioactive compounds. For &lt;em&gt;in vivo&lt;/em&gt; validation, rats were treated with single extracts (300 mg/kg bw) or combinations (ratios 1:1, 1:3, 3:1), followed by isoproterenol-induced hypercoagulability. Anticoagulant activity was assessed by measuring Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT).&lt;strong&gt; Results:&lt;/strong&gt; Network pharmacology identified 10 key hub proteins, including STAT3, AKT1, and MTOR, indicating a multi-target mechanism. &lt;em&gt;In vivo&lt;/em&gt;, isoproterenol effectively induced hypercoagulability. While ZOR (300 mg/kg BW) showed the highest individual potency, all combinations significantly prolonged PT and aPTT (p &amp;lt; 0.05). Based on Chou-Talalay analysis, the interaction was dose-dependent; PT showed nearly additive to synergistic effects (CI: 0.86– 1.07), whereas aPTT exhibited antagonistic trends (CI: 1.06–1.50) at higher ZOR ratios. Consequently, combination effects remained stable without exceeding the most potent single dose. &lt;strong&gt;Conclusion: &lt;/strong&gt;The ZOR and CL combination provides a consistent anticoagulant response through additive interactions, likely mediated by the modulation of the AKT/mTOR signaling axis and coagulation factors. This integration offers a promising, safer multi-target strategy for preventing thrombotic events in myocardial infarction.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">139</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Puspa SD. Solihah&lt;sup&gt;1*&lt;/sup&gt;, Ellisa Clara Pasaribu&lt;sup&gt;1&lt;/sup&gt;, Fahmi Ahsanul Haq&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy, Universitas Jenderal Achmad Yani (UNJANI), Jalan Terusan Jenderal Sudirman, Cimahi, West Java, INDONESIA.&lt;/p&gt;
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